Leishmaniasis APP: Leishmaniasis parasite detection & analysis in cell culture and tissue
The APP was designed to assist in immunology research into Leishmaniasis and can be adapted for research on other intracellular parasites. It can be used both on cell cultures and tissue sections. Its main function is to automatically detect parasite stages in cell compartments and quantify them in context with specific immune markers. The parasite load and the status of parasite stages, e.g. “live/dead” can also be evaluated and all results can be output.
Cell detection with specific live/dead parasite stage combinations
Mouse skin-draining lymph node stained with DAPI (nucelus, blue) and resulting epithelial area detection