Checkpoint Molecules Predict Response to Immunotherapy in Cancer

FEATURED PUBLICATION

Checkpoint Molecules Predict Response to Immunotherapy in Cancer
cells

Heij et al. investigated the expression of checkpoint molecules in the tumor microenvironment of intrahepatic cholangio-carcinoma. They found that co-expression patterns of checkpoint molecules such as PD-L1 and TIGIT correlate with metastases and poor prognosis. TissueFAXS and StrataQuest were used to image the mIF-stained samples and assess protein expression.

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Community-developed Checklists Enhance Reproducibility of Microscopy Images

FEATURED PUBLICATION

Community-developed checklists for publishing images and image analyses
Nature Methods

QUAREP-LiMi is an initiative focused on promoting quality assessment and reproducibility for instruments and images in light microscopy. Dr. Rupert Ecker, CEO of TissueGnostics, participated in developing standardized guidelines for best practices in image communication. These checklists offer key recommendations on image formatting and annotation among others.

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AI-Assisted Analysis of ‘Anti-/Pro-Tumor’ Profiles for Pancreatic Adenocarcinoma

FEATURED PUBLICATION

AI-Assisted Analysis of ‘Anti-/Pro-Tumor’ Profiles for Pancreatic Adenocarcinoma
Cancer Biology & Medicine

To predict survival of patients with pancreatic adenocarcinoma, Zhou et al. performed an AI-assisted comprehensive analysis of CD8+ T cells, cancer stem cells (CSCs), and tumor budding (TB). They used StrataQuest image analysis software to automatically detect and quantify tumor-infiltrating CD8+ T cells, CD133+ CSCs, and CK19+ TB.

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Characterization of the Spatial Immune Context of Lung Cancer

FEATURED PUBLICATION

Characterization of the Spatial Immune Context of Lung Cancer
International Journal of Molecular Sciences

In a recent study, Imamura et al. investigated the role of debrin-expressing tumor infiltration lymphocytes (TIL) in lung cancer. The high-end image analysis solution, StrataQuest, was used for the automatic tissue classification and localization of CD3+/debrin+ cellular phenotypes in the tumor cell nest, as well as in the stroma.

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COL8A1 Promotes Tumor in Pancreatic Ductal Adenocarcinoma

FEATURED PUBLICATION

COL8A1 Promotes Tumor in Pancreatic Ductal Adenocarcinoma
Matrix Biology

Yan et al. investigated several collagen subtypes involved in pancreatic ductal adenocarcinoma desmoplasia. Based on this study, COL8A1 was suggested as a new marker for predicting poor chemotherapy responsiveness and survival. The TissueFAXS imaging platform and StrataQuest analysis solution were used to image and segment morphological structures.

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Erk1/2-Dependent HNSCC Cell Susceptibility to Erastin-Induced Ferroptosis

FEATURED PUBLICATION

Erk1/2-Dependent HNSCC Cell Susceptibility to Erastin-Induced Ferroptosis
cells

Savic et al. investigated the Erk 1/2 pathway role in ferroptosis induction by targeting xCT protein during the treatment of HNSCC. They found erastin to be a potent ferroptosis inducer which is strongly dependent on Erk 1/2 kinases. TissueFAXS and HistoQuest were used to image the IHC-stained samples and calculate the percentage of xCT-positive cells.

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Tumor Microenvironment Characterization of Rectal Cancer

FEATURED PUBLICATION

Case Report: Tumor Microenvironment Characterization of Rectal Cancer
Frontiers in Immunology

Song et al. published a case study investigating the genetics and tumor microenvironment of a rectal cancer patient with MSS/PD-L1-negative recurrent hepatopulmonary metastasis. TissueFAXS and StrataQuest were used to image stained tissue samples and to assess tumor infiltrating immune cells using, among others, CD4, CD8, CD163, PD1, and PD-L1 as markers.

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Adverse Effects of Single

FEATURED PUBLICATION

The impact of HIV infection on the frequencies, function, spatial localization and heterogeneity of TFRs within human lymph nodes
BMC Immunology

Mahlobo et al. published a study in BMC Immunology addressing the effect of HIV infections on frequencies, function, spatial localization and heterogeneity of T follicular regulatory cells (TFRs) in human lymph nodes. TFRs within lymph node tissues were quantified using TissueFAXS i PLUS and TissueQuest.

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Temporal changes

FEATURED PUBLICATION

Temporal changes in T cell subsets and expansion of cytotoxic CD4+ T cells in the lungs in severe COVID-19
Clinical Immunology

Kaneko et al. published a study in the Clinical Immunology journal focusing on the quantitative analysis of T-cell subset alterations in thoracic lymph nodes and lung samples of COVID-19 patients. With the help of TissueFAXS and StrataQuest, the authors were first to show the expansion of cytotoxic CD4+ T cells in the lungs of patients with severe COVID-19.

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Spatial Distribution

FEATURED PUBLICATION

Spatial distribution and functional analysis define the action pathway of Tim-3/Tim-3 ligands in tumor development
Molecular Therapy

In a recent publication in the journal Molecular Therapy, Wang et al (Cheeloo Medical College of Shandong University) combine multiplexing and TissueGnostics’ image cytometry solutions for the assessment of the expression level of Tim-3, a well-known immune checkpoint, in tumor infiltrating lymphocytes. This study nominates Tim-3 as a potential target for cancer immunotherapy.

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Multi-Antigen Imaging

FEATURED PUBLICATION

Multi-Antigen Imaging Reveals Inflammatory DC, ADAM17 and Neprilysin as Effectors in Keloid Formation
International Journal of Molecular Sciences

In a recent publication in the MDPI - International Journal of Molecular Sciences, Rath et al (University Hospital Heidelberg) combine multiplexing, TissueGnostics’ image cytometry and in-depth data mining to characterize the immune microenvironment including spatial relationships within keloid lesions. Using this approach, the authors were able to nominate potential therapeutic targets.

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SynNotch-CAR T

FEATURED PUBLICATION

SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma
Science Translational Medicine

A recent publication from the group of Choe et al. (University of California, UCSF) published in Science Translational research is exploring the therapeutic effectiveness of SynNotch-CAR T cells in a PDX glioblastoma mouse model. T cells in-situ were imaged/assessed by TissueFAXS and quantified via StrataQuest.

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CpG Methylation Influence on Protease Serine 3 Isoforms in Lung Cancer

FEATURED PUBLICATION

CpG Methylation Influence on Protease Serine 3 Isoforms in Lung Cancer
Acta Pharmaceutica Sinica B

Lin et al. investigated the UHRF1/DNMT1–MZF1 axis in the context of CpG methylation of protease serine 3, a protein with several isoforms that show paradoxical effects. To differentiate the isoforms, the researchers employed 7-marker multiplex staining and performed a quantitative analysis using TissueFAXS SPECTRA and StrataQuest.

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Customer Publications (A total of 3219 papers published)

Results 1 - 10 of 3219
01
Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation Runch

XJ Liu, YB Jia, CH Wu, XX Wang, KM Yang, X Yao, JH Ling

World Journal of Gastroenterology (Baisideng Publishing Group) China , 07 March 2025

Gastroenterology

Organism: Human

Tissue: Colon

Pathological State: Functional Constipation

02
cGAMP promotes inner blood-retinal barrier breakdown through P2RX7-mediated transportation into microglia

X Ge, X Zhu, W Liu, M Li, Z Zhang, M Zou, M Deng, H Cui, Z Chen, L Wang, X Hu, R Ju, X Tang, X Ding, L Gong

Journal of Neuroinflammation (BMC) China , 01 March 2025

Neuroscience

Organism: Mouse

Tissue: Eyeball

03
The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation

T Neuwirth, D Malzl, K Knapp, P Tsokkou, L Kleissl, A Gabriel, B Reininger, C Freystätter, N Marella, AP Kutschat, E Ponweiser, A Haschemi, D Seruggia, J Menche, EF Wagner, G Stary

Immunity (Cell) Austria , 28 February 2025

Dermatology

Key words: regulatory T cells, chronic inflammation, polyamine metabolism, psoriasis

Organism: Mouse

Tissue: Skin

Pathological State: Inflammation

04
The Protective Effects of Perch Essence Against Muscle Atrophy in Cancer Cachexia and Cisplatin Treatment

SL Yeh, PY Chen, JW Liao, RL Huang, SH Yu, LN Chen, MH Lee, LW Chen, HW Chen, YC Yang, YL Wu, KL Liu

Current Issues in Molecular Biology (MDPI) Taiwan , 26 February 2025

Oncology

Key words: perch essence; cancer cachexia; cisplatin; muscle atrophy

Organism: Mouse

Tissue: Muscle

Pathological State: Cacner

05
Cecelia: a multifunctional image analysis toolbox for decoding spatial cellular interactions and behaviour

D Schienstock, JL Hor, S Devi, SN Mueller

Nature Communications (Nature) Australia , 24 February 2025

Medical Image Analysis

Key words: Fluorescence imaging, Imaging the immune system, Software

06
PCSK9 Enhances Cardiac Fibrogenesis via the Activation of Toll-like Receptor and NLRP3 Inflammasome Signaling

CC Chung, YH Kao, YC Chen, YK Lin, S Higa, KC Hsu, YJ Chen

International Journal of Molecular Science (MDPI) Taiwan, Japan , 23 February 2025

Cardiology

Key words: fibroblasts; fibrosis; PCSK9; NLRP3; toll-like receptor; inflammasome

Organism: Human

Tissue: Heart

Pathological State: Fibrogenesis

07
IL35 modulates HBV-related HCC progression via IL6-STAT3 signaling

M Zhou, Y Xia, S Wang

Scientific Reports (Nature) China , 21 February 2025

Oncology

Key words: HCC, HBV, IL35, IL6-STAT3

Organism: Human

Tissue: Liver

Pathological State: Cancer

08
Streamlined Quantification of p-γ-H2AX Foci for DNA Damage Analysis in Melanoma and Melanocyte Co-cultures Exposed to FLASH Irradiation Using Automated Image Cytometry

S Orobeti, I Dinca, A Bran, I Tiseanu, F Sima, SM Petrescu, LE Sima

Bio Protocol (Bio Protocol) Romania , 20 February 2025

Medical Image Analysis

Key words: Cancer cells, FLASH LPA electrons cell irradiation, DNA damage, Immunofluorescence, γ-H2AX foci, Image cytometry

Organism: Human

Pathological State: Cancer

Cell Line: A375

09
Current advances in precision microscopy

RC Ecker, F Mungenast, Q Nguyen, J Batra

Frontiers in Medicine (Frontiersin) Australia, Austria , 18 February 2025

Medical Imaging

Key words: precision microscopy, image cytometry, organoid, single cell analysis, multiplexing, intravital microscopy, deep learning, machine learning

10
Targeting TNIP1 as a new therapeutic avenue for major depressive disorder

YY Hung, CY Tsai, CT Lee, HC Fu, CK Chou, YC Yang, JF Chen, HY Kang

Brain, Behavior, and Immunity (Elsevier) Taiwan , 18 February 2025

Neuroscience

Key words: Neuroinflammation, Antidepressants, Hippocampus, PPAR-γ, Duloxetine

Organism: Mouse

Tissue: Brain

Pathological State: Viral Infection

Results 1 - 10 of 3219
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