White Papers

Quantitative confocal imaging helps researchers to confirm muscle protein restoration after Cas9 mRNA delivery via targeted lipid nanoparticles.

Quantitative cellular analysis of COVID-19-infected astrocyte cells reveals a more central role of astrocytes in NeuroCOVID-19 and the interruptive impact of SARS-CoV-2 on astrocyte homeostasis capabilities, reframing astrocytes as active protagonists.

A recent study, published in Clinical and Translational Medicine, provides new insights into the colorectal cancer (CRC) immune macroenvironment, identifying key clinical biomarkers that could help stratify CRC patients for immune checkpoint inhibitor (ICI) therapies.

A recent study uncovered how loss of the tumor suppressor FRMD8 contributes to tamoxifen resistance in breast cancer. Multiplex immunofluorescence and automated image analysis revealed reduced ERα and PR expression and highlighted changes in the tumor microenvironment.

The evolving understanding of cancer biology underscores the pivotal role of epigenetic modifications - heritable yet reversible changes in gene expression that do not alter the DNA sequence.

A new study shows PD-1+ Treg/CTL ratio combined with TLS predicts prognosis and treatment response in gastric cancer using mIF, TissueFAXS, and StrataQuest.

Researchers from Soochow University investigated the B7-H4/USP2a axis in lung cancer using TissueFAXS Spectra for TMA imaging and StrataQuest for protein expression analysis.

In HNSCC, CD8+ T-cell infiltration is often impaired. Chuwen Li et al. used mIF, TissueFAXS Spectra, and StrataQuest to image and analyze the spatial distribution of Gal9+ CAFs and CD8+ T cells, complementing prior spatial transcriptomics.

Researchers from the Medical University of Vienna developed an automated algorithm in StrataQuest to detect and quantify osteoclasts in cell culture. The workflow replaces manual counting of TRAP-positive multinucleated cells with automated image analysis, providing faster, reproducible measurements of osteoclast number, nuclei count, and cell area for bone research and drug screening studies.

Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin, confirmed by multiplex immunostaining with TissueFAXS Spectra imaging platform, and StrataQuest image analysis software was employed for marker identification.

The StrataQuest Organoid App provides reliable and efficient high-throughput validation and quantification for organoids co-cultured alongside immune cells.

An interview with Dr. Nidhi Dey explores how spatial analysis of immune checkpoints IDO1 and PDL1 in cutaneous leishmaniasis reveals interactions between infected macrophages and T cells. The findings highlight new biomarkers and potential targets for host-directed therapies.

A recent study used TissueFAXS and StrataQuest tissue cytometry to investigate cytolytic CD8+ T cells in lymph nodes of spontaneous HIV controllers. Spatial analysis revealed clusters of perforin- and granzyme B–expressing CD8+ cells near HIV RNA, highlighting mechanisms of immune-mediated viral control.

A rare MSS-type CRC patient responded to immunotherapy. TissueFAXS SL and StrataQuest were used to image and quantify immune phenotypes.

A new study shows that combining type I interferon (MxA) expression with CD8+ T cell density can predict patient response to neoadjuvant treatment in rectal adenocarcinoma. TissueFAXS CHROMA and StrataQuest enabled precise multispectral imaging and analysis.

A recent study used StrataQuest image cytometry to investigate the role of the histone demethylase KDM1A in chronic lymphocytic leukemia. Automated analysis of murine spleen sections revealed its impact on B-cell proliferation, apoptosis, and the tumor microenvironment.

A recent study using TissueFAXS and HistoQuest revealed that xCT inhibition by erastin may trigger ferroptosis in HNSCC cells. Erk1/2 activation sensitized tumor cells to this process, suggesting novel therapeutic strategies for head and neck cancer.

Researchers from Sichuan University performed transcriptomic and multiplex immunofluorescence analyses of glioma samples using TissueFAXS SPECTRA and StrataQuest. Findings highlight B7-H3 as a prognostic biomarker and potential immunotherapy target in glioma.

A study using TissueFAXS CHROMA and StrataQuest revealed that CD103+CD39+CD8+ TRM cells accumulate in colorectal liver metastases compared to adjacent liver tissue. These findings highlight their potential as therapeutic targets in advanced colorectal cancer.

This white paper details the first in-depth tissue cytometry analysis of CD3+ T-cell subsets in COVID-19 lungs. Using TissueFAXS PLUS and StrataQuest, researchers uncovered dysfunctional CD8+ T cells and expansion of cytotoxic CD4+ T cells driving impaired immunity.