Researching the Role of Immune Checkpoints, IDO1 and PDL1, to Study Cutaneous Leishmaniasis
In this interview, Dr. Nidhi S. Dey, University of York, presents her exciting new work on the spatial analysis of IDO1 and PDL1 checkpoints in cutaneous leishmaniasis.
Characterizing the Tumor Microenvironment of Colorectal Cancer Patients
A 50-year-old patient with MSS-type CRC and PD-L1-negative recurrent hepatopulmonary metastases underwent complete remission and prolonged benefits from immunotherapy treatments after systematic treatments had previously failed.
Type I interferon and CD8+ T cell detection help to predict the response to neoadjuvant treatment of rectal adenocarcinoma
A recent study discovered that the equal weighing of two separate quantifications of type 1 interferon (IFN) expression and CD8+ T cell density is capable of predicting the chances of patients displaying a pathological complete response to neoadjuvant treatment of rectal adenocarcinoma.
Beyond Genetics: Exploring the Role of Epigenetic Modification in Chronic Lymphocytic Leukemia
In chronic lymphocytic leukemia (CLL), research has shown that epigenetic changes are a major determinant of the different biological features of the disease. In a new study, the researchers employed TissueGnostics´ StrataQuest image cytometry software to compute and analyze murine spleen sections, helping to reveal the impact of KDM1A on B cell proliferation and apoptosis.
Activated Erk 1/2 kinases decrease cell viability caused by erastin in HNSCC
Using TissueFAXS whole-slide imaging and HistoQuest single cell analysis solution, a recent study revealed that xCT inhibition, caused by erastin, may be a promising therapeutic approach for treating HNSCC tumorous cells through the initiation of cell ferroptosis.
In-Depth Immune Checkpoint Analysis of Glioma at Transcript and Protein Level
Glioma is the most common type of malignant brain tumor and is associated with high morbidity and mortality. To better understand the relationship between phenotype and transcript-level changes in glioma, the team looked at protein expression in glioma samples from 168 patients, using TissueFAXS SPECTRA and StrataQuest.
TissueFAXS Chroma and StrataQuest analysis to identify potential targets for therapies against colorectal liver metastases
Colorectal cancer is the second most common cause of cancer-related mortality. One novel approach in cancer treatment is to target the anti or pro tumoral immune response. It has been reported that CD103+CD39+CD8+ TRM cells are present at higher levels in CRLM relative to adjacent liver tissue. These specific subpopulations of CD8 cells have now been evaluated in detail using the TissueFAXS CHROMA imaging platform and StrataQuest image analysis software.
Comprehensive Tissue Cytometry Analysis of CD3+ T cells in Lungs of COVID-19 Patients
The appearance of a novel highly infectious human coronavirus (SARS-CoV-2) in 2019 resulted in a global pandemic that caused social and economic devastation worldwide. Infection with SARS-CoV-2 has a highly heterogenous presentation but can lead to life-threatening respiratory failure, even in otherwise healthy individuals.
IBEX and StrataQuest image analysis compatibility
One of the challenges of imaging tissue sections is dealing with their diverse composition. The human body contains hundreds of different cell phenotypes, with many more to be discovered. There is a huge need for multiplexing imaging techniques that can recover imaging information on cell-cell interactions with sufficient resolution to identify various unique phenotypes and is compatible with the diversity of cell species.
PD-L1 raises new hope in the treatment of cutaneous leishmaniasis
Cutaneous leishmaniasis (CL) caused by infection with the Leishmania protozoan is endemic in Sri Lanka. The disease is transmitted by the sandfly and causes widespread burden due to the social stigma causing sufferers to be ostracized and forced into financial hardship. Treatments for cutaneous leishmaniasis have limited success and so, once contracted, patients often face a lifetime of misery.
Enhancing CAR-T Cell Therapy for Glioblastoma
Surgery, chemotherapy and radiation have formed the mainstay of cancer therapy for many years. However, in addition to destroying tumor cells, these treatments are detrimental to healthy cells giving rise to side effects, which can be severe. During the last two decades, more targeted therapies that specifically destroy cancer cells have been developed to minimize unwanted side effects.
Redefine the Transcriptional and Epigenetic Signature of Tissue-associated Regulatory T Cells
Recently Delacher and colleagues were able to redefine the transcriptional and epigenetic signature of tissue-associated regulatory T cells (Treg) in mice and humans (Delacher et al. 2021). They could demonstrate, that Treg cells from healthy tissues share distinct expression patterns with tumor-infiltrating Treg cells...
Evaluating the Nature of Immune Responses to Implanted Polypropylene Mesh
Surgical mesh is used in a range of tissue repair procedures to stabilize and strengthen soft tissue defects or to support prolapsed organs and viscera. One of their most common applications is hernia repair. The mesh serves to mechanically strengthen the weakened area while simultaneously providing a scaffold to support the growth of new tissue...
Understanding HCC Development and Progression
Liver cancer is the fourth leading cause of cancer-related death worldwide, of which hepatocellular carcinoma (HCC) is the most common primary cancer accounting for 75-85% of cases. HCC can be classified into several histological subtypes, reflecting the heterogeneity of the disease. This heterogeneity, including the cancers’ cell plasticity, is known to be, in part, determined by the microenvironment.
Striosomes Causal Changes Task Engagement
Learning through affective responses, in which experiences are regarded as having a positive or negative valence (e.g., pleasure or fear, respectively), requires a judgment of the relative value of options. Several studies have linked a cluster of neurons in the subcortical basal ganglia, known as the striatum, to learning these valence-based responses.
Mediating Palatability Guided Food and Water Consumption with Hindbrain Double Negative Feedback
Animal survival relies on neural mechanisms that trigger behaviors to meet basic bodily needs. Traditionally, motivated behaviors have been categorized into three phases.
COVID-19 Shows a Loss of Bcl6 Expressing T Follicular Helper Cells and Germinal Centers
A groundbreaking publication in the journal Cell suggests that in COVID-19 patients the differentiation of Tfh cells and formation of germinal centers within lymph nodes and spleen is inhibited. Molecules such as Bcl-6 were assessed using the TissueFAXS platform including the analysis applications StrataQuest and TissueQuest.
Characterizing the Complex Microenvironment of Individual Immune Cells
Research groups from Yale University, USA published a paper in 'Cytometry' focusing on the phenotyping, quantification and visualization of immune cells and immune cell clusters within tissue sections using co-registration and IF multiplexed tissue cytometry.
Imaging of neural contributions to voluntary hand movement
Skilled motor behavior requires the precise exchange of information between the muscles of the body and various levels of the central nervous system (CNS). Motor behavior, limb mechanics and neural control must be precisely coordinated in order to achieve the required movement.
DNA-Binding of the Tet-Transactivator Curtails Antigen-Induced Lymphocyte Activation in Mice
Research groups from Austria, Australia, England and The Netherlands published a paper in 'Nature Communications' about the Tet-transactivator imparing the antigen-induced lymphocyte activation in mice.
Tumor Specific Regulatory T Cells in the Bone Marrow of Breast Cancer Patients Selectively Upregulate the Emigration Receptor S1P1
Groups from Germany (Heidelberg, Jena) published a paper in the Journal 'Cancer Immunology, Immunotherapy' regarding tumor-specifc regulatory T cells in the bone marrow of breast cancer patients.
Quantitative Comparison and Reproducibility of Pathologist Scoring and Digital Image Analysis of Estrogen Receptor β2 Immunohistochemistry in Prostate Cancer
Research groups from USA, published a paper in 'Diagnostic Pathology' dealing with quantitative comparison and reproducibility of pathologist scoring and digital image analysis in prostate cancer.
Anti-Neoplastic Effects of Calcium in Colorectal Cancer Potentiated by Active Vitamin D
Groups from Vienna published a paper in 'The Journal of Steroid Biochemistry and Molecular Biology' about Vitamin D potentiating the anti-neoplastic effects of calcium in the colon.
The Role of Cytolytic CD8+ T Cells in Suppressing Ongoing HIV Replication in Spontaneous Controller Lymph Nodes
Using TissueGnostics’ tissue cytometry solutions, a recent study revealed important information on how sustained cytolytic CD8+ T cells are central in determining long-term viral control in lymph nodes (LNs).