White Papers

Quantitative cellular analysis of COVID-19-infected astrocyte cells reveals a more central role of astrocytes in NeuroCOVID-19 and the interruptive impact of SARS-CoV-2 on astrocyte homeostasis capabilities, reframing astrocytes as active protagonists.

A recent study, published in Clinical and Translational Medicine, provides new insights into the colorectal cancer (CRC) immune macroenvironment, identifying key clinical biomarkers that could help stratify CRC patients for immune checkpoint inhibitor (ICI) therapies.

A recent study uncovered how loss of the tumor suppressor FRMD8 contributes to tamoxifen resistance in breast cancer. Multiplex immunofluorescence and automated image analysis revealed reduced ERα and PR expression and highlighted changes in the tumor microenvironment.

The evolving understanding of cancer biology underscores the pivotal role of epigenetic modifications - heritable yet reversible changes in gene expression that do not alter the DNA sequence.

A new study shows PD-1+ Treg/CTL ratio combined with TLS predicts prognosis and treatment response in gastric cancer using mIF, TissueFAXS, and StrataQuest.

Researchers from Soochow University investigated the B7-H4/USP2a axis in lung cancer using TissueFAXS Spectra for TMA imaging and StrataQuest for protein expression analysis.

In HNSCC, CD8+ T-cell infiltration is often impaired. Chuwen Li et al. used mIF, TissueFAXS Spectra, and StrataQuest to image and analyze the spatial distribution of Gal9+ CAFs and CD8+ T cells, complementing prior spatial transcriptomics.

Researchers from the Medical University of Vienna developed an automated algorithm in StrataQuest to detect and quantify osteoclasts in cell culture. The workflow replaces manual counting of TRAP-positive multinucleated cells with automated image analysis, providing faster, reproducible measurements of osteoclast number, nuclei count, and cell area for bone research and drug screening studies.

Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin, confirmed by multiplex immunostaining with TissueFAXS Spectra imaging platform, and StrataQuest image analysis software was employed for marker identification.

The StrataQuest Organoid App provides reliable and efficient high-throughput validation and quantification for organoids co-cultured alongside immune cells.

An interview with Dr. Nidhi Dey explores how spatial analysis of immune checkpoints IDO1 and PDL1 in cutaneous leishmaniasis reveals interactions between infected macrophages and T cells. The findings highlight new biomarkers and potential targets for host-directed therapies.

A recent study used TissueFAXS and StrataQuest tissue cytometry to investigate cytolytic CD8+ T cells in lymph nodes of spontaneous HIV controllers. Spatial analysis revealed clusters of perforin- and granzyme B–expressing CD8+ cells near HIV RNA, highlighting mechanisms of immune-mediated viral control.

A rare MSS-type CRC patient responded to immunotherapy. TissueFAXS SL and StrataQuest were used to image and quantify immune phenotypes.

A new study shows that combining type I interferon (MxA) expression with CD8+ T cell density can predict patient response to neoadjuvant treatment in rectal adenocarcinoma. TissueFAXS CHROMA and StrataQuest enabled precise multispectral imaging and analysis.

A recent study used StrataQuest image cytometry to investigate the role of the histone demethylase KDM1A in chronic lymphocytic leukemia. Automated analysis of murine spleen sections revealed its impact on B-cell proliferation, apoptosis, and the tumor microenvironment.

A recent study using TissueFAXS and HistoQuest revealed that xCT inhibition by erastin may trigger ferroptosis in HNSCC cells. Erk1/2 activation sensitized tumor cells to this process, suggesting novel therapeutic strategies for head and neck cancer.

Researchers from Sichuan University performed transcriptomic and multiplex immunofluorescence analyses of glioma samples using TissueFAXS SPECTRA and StrataQuest. Findings highlight B7-H3 as a prognostic biomarker and potential immunotherapy target in glioma.

A study using TissueFAXS CHROMA and StrataQuest revealed that CD103+CD39+CD8+ TRM cells accumulate in colorectal liver metastases compared to adjacent liver tissue. These findings highlight their potential as therapeutic targets in advanced colorectal cancer.

This white paper details the first in-depth tissue cytometry analysis of CD3+ T-cell subsets in COVID-19 lungs. Using TissueFAXS PLUS and StrataQuest, researchers uncovered dysfunctional CD8+ T cells and expansion of cytotoxic CD4+ T cells driving impaired immunity.

The IBEX protocol enables high-content multiplex imaging with hundreds of antibodies. When paired with StrataQuest, large IBEX datasets can be rapidly aligned, normalized, and analyzed for tissue classification, immunophenotyping, and spatial analysis.

PD-L1 expression has emerged as a predictor of treatment response in cutaneous leishmaniasis. Using StrataQuest image analysis, researchers quantified immune markers and parasites, highlighting PD-L1 as a potential host-directed therapeutic target.

Researchers developed advanced synNotch-CAR T cells to improve immunotherapy for glioblastoma by targeting multiple tumor antigens. TissueFAXS and StrataQuest were used to analyze immunofluorescence data, supporting the evaluation of CAR-T cell responses in tumor tissue.

A study by Delacher et al. redefined the transcriptional and epigenetic signature of tissue-associated regulatory T cells. Advanced imaging and automated tissue segmentation enabled precise quantification of epidermal layers during wound healing in 3D skin models.

Researchers analyzed immune responses to implanted polypropylene surgical meshes using multiplex immunofluorescence imaging. The study revealed that both innate and adaptive immune cells—particularly macrophages and T cells—contribute to the foreign body reaction around mesh fibers.

A study on hepatocellular carcinoma (HCC) revealed that imbalance between the TGF-β and BMP signaling pathways drives tumor progression and poor clinical outcomes. Multispectral immunofluorescence imaging enabled in-situ analysis of pathway proteins in liver tumor tissue.

A study in Cell demonstrated that striosomes in the striatum causally regulate task engagement during value-based learning. High-throughput confocal imaging and multiplex staining revealed how striosomal activity influences motivation and decision-making.

Researchers identified hindbrain neurons that integrate hunger and thirst signals to regulate food and water intake. Brain-wide imaging and automated cell quantification revealed that periLC neurons control palatability during the consummatory phase of ingestive behavior.

A Cell study revealed impaired germinal center formation and reduced Bcl-6–expressing T follicular helper cells in COVID-19 patients. Multispectral imaging and multiplex immunofluorescence were used to analyze lymph nodes and spleen architecture at single-cell resolution.

Researchers from Yale University used multiplex immunofluorescence and tissue cytometry to characterize immune cell phenotypes and clusters in situ. TissueFAXS and StrataQuest enabled multiplex biomarker analysis while preserving spatial context within tissue sections.

Researchers quantified sensory and motor axons in the human brachial plexus using ChAT and neurofilament immunofluorescence. Automated whole-slide analysis revealed that sensory axons vastly outnumber motor axons, providing new insights into the neural control of fine hand movements.

A Nature Communications study investigated how DNA binding of the Tet-transactivator affects antigen-induced lymphocyte activation in mice. Histological analysis of spleen sections and quantitative image cytometry revealed reduced survival of activated T and B cells.

A study in Cancer Immunology, Immunotherapy investigated tumor-specific regulatory T cells in breast cancer patients. Multicolor immunofluorescence analysis revealed that Treg cells upregulate the emigration receptor S1P1, promoting their migration from bone marrow to tumor tissue.

A study in Diagnostic Pathology compared traditional pathologist scoring with digital image analysis for ERβ2 immunohistochemistry in prostate cancer tissue microarrays. Digital analysis demonstrated higher reproducibility and improved quantitative assessment of biomarker expression.

A study from Vienna explored how active vitamin D enhances the anti-neoplastic effects of calcium in colorectal cancer. Quantitative analysis of Ki67-stained colon sections revealed reduced proliferation and highlighted the role of the calcium-sensing receptor in CRC prevention.