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Please find a curated selection of research made possible by TissueGnostics software and systems below or in our searchable data base.

 

  • Cytolytic CD8+ T Cells

    The Role of Cytolytic CD8+ T Cells in Suppressing Ongoing HIV Replication in Spontaneous Controller Lymph Nodes

    November, 2023

    Using TissueGnostics’ tissue cytometry solutions, a recent study revealed important information on how sustained cytolytic CD8+ T cells are central in determining long-term viral control in lymph nodes (LNs).

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  • Tumor Microenvironment

    Researching the Role of Immune Checkpoints, IDO1 and PDL1, to Study Cutaneous Leishmaniasis

    November, 2023

    In this interview, Dr. Nidhi S. Dey, University of York, presents her exciting new work on the spatial analysis of IDO1 and PDL1 checkpoints in cutaneous leishmaniasis.

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  • Tumor Microenvironment

    Characterizing the Tumor Microenvironment of Colorectal Cancer Patients

    October, 2023

    A 50-year-old patient with MSS-type CRC and PD-L1-negative recurrent hepatopulmonary metastases underwent complete remission and prolonged benefits from immunotherapy treatments after systematic treatments had previously failed. 

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  • Polypropylene Mesh

    Type I interferon and CD8+ T cell detection help to predict the response to neoadjuvant treatment of rectal adenocarcinoma

    October, 2023

    A recent study discovered that the equal weighing of two separate quantifications of type 1 interferon (IFN) expression and CD8+ T cell density is capable of predicting the chances of patients displaying a pathological complete response to neoadjuvant treatment of rectal adenocarcinoma.

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  • Polypropylene Mesh

    Beyond Genetics: Exploring the Role of Epigenetic Modification in Chronic Lymphocytic Leukemia

    July, 2023

    In chronic lymphocytic leukemia (CLL), research has shown that epigenetic changes are a major determinant of the different biological features of the disease. In a new study, the researchers employed TissueGnostics´ StrataQuest image cytometry software to compute and analyze murine spleen sections, helping to reveal the impact of KDM1A on B cell proliferation and apoptosis.

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  • Polypropylene Mesh

    Activated Erk 1/2 kinases decrease cell viability caused by erastin in HNSCC

    June, 2023

    Using TissueFAXS whole-slide imaging and HistoQuest single cell analysis solution, a recent study revealed that xCT inhibition, caused by erastin, may be a promising therapeutic approach for treating HNSCC tumorous cells through the initiation of cell ferroptosis.

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  • Polypropylene Mesh

    In-Depth Immune Checkpoint Analysis of Glioma at Transcript and Protein Level

    February, 2023

    Glioma is the most common type of malignant brain tumor and is associated with high morbidity and mortality. To better understand the relationship between phenotype and transcript-level changes in glioma, the team looked at protein expression in glioma samples from 168 patients, using TissueFAXS SPECTRA and StrataQuest.

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  • Polypropylene Mesh

    TissueFAXS Chroma and StrataQuest analysis to identify potential targets for therapies against colorectal liver metastases

    October, 2022

    Colorectal cancer is the second most common cause of cancer-related mortality. One novel approach in cancer treatment is to target the anti or pro tumoral immune response. It has been reported that CD103+CD39+CD8+ TRM cells are present at higher levels in CRLM relative to adjacent liver tissue. These specific subpopulations of CD8 cells have now been evaluated in detail using the TissueFAXS CHROMA imaging platform and StrataQuest image analysis software.

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  • Polypropylene Mesh

    Comprehensive Tissue Cytometry Analysis of CD3+ T cells in Lungs of COVID-19 Patients

    June, 2022

    The appearance of a novel highly infectious human coronavirus (SARS-CoV-2) in 2019 resulted in a global pandemic that caused social and economic devastation worldwide. Infection with SARS-CoV-2 has a highly heterogenous presentation but can lead to life-threatening respiratory failure, even in otherwise healthy individuals.

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  • Polypropylene Mesh

    IBEX and StrataQuest image analysis compatibility

    May, 2022

    One of the challenges of imaging tissue sections is dealing with their diverse composition. The human body contains hundreds of different cell phenotypes, with many more to be discovered. There is a huge need for multiplexing imaging techniques that can recover imaging information on cell-cell interactions with sufficient resolution to identify various unique phenotypes and is compatible with the diversity of cell species.

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  • Polypropylene Mesh

    PD-L1 raises new hope in the treatment of cutaneous leishmaniasis

    January, 2022

    Cutaneous leishmaniasis (CL) caused by infection with the Leishmania protozoan is endemic in Sri Lanka. The disease is transmitted by the sandfly and causes widespread burden due to the social stigma causing sufferers to be ostracized and forced into financial hardship. Treatments for cutaneous leishmaniasis have limited success and so, once contracted, patients often face a lifetime of misery.

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  • Polypropylene Mesh

    Enhancing CAR-T Cell Therapy for Glioblastoma

    October, 2021

    Surgery, chemotherapy and radiation have formed the mainstay of cancer therapy for many years. However, in addition to destroying tumor cells, these treatments are detrimental to healthy cells giving rise to side effects, which can be severe. During the last two decades, more targeted therapies that specifically destroy cancer cells have been developed to minimize unwanted side effects.

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  • Polypropylene Mesh

    Redefine the Transcriptional and Epigenetic Signature of Tissue-associated Regulatory T Cells

    September, 2021

    Recently Delacher and colleagues were able to redefine the transcriptional and epigenetic signature of tissue-associated regulatory T cells (Treg) in mice and humans (Delacher et al. 2021). They could demonstrate, that Treg cells from healthy tissues share distinct expression patterns with tumor-infiltrating Treg cells...

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  • Polypropylene Mesh

    Evaluating the Nature of Immune Responses to Implanted Polypropylene Mesh

    July, 2021

    Surgical mesh is used in a range of tissue repair procedures to stabilize and strengthen soft tissue defects or to support prolapsed organs and viscera. One of their most common applications is hernia repair. The mesh serves to mechanically strengthen the weakened area while simultaneously providing a scaffold to support the growth of new tissue...

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  • Anti-Neoplastic Effects of Calcium

    Understanding HCC Development and Progression 

    May, 2021

    Liver cancer is the fourth leading cause of cancer-related death worldwide, of which hepatocellular carcinoma (HCC) is the most common primary cancer accounting for 75-85% of cases. HCC can be classified into several histological subtypes, reflecting the heterogeneity of the disease. This heterogeneity, including the cancers’ cell plasticity, is known to be, in part, determined by the microenvironment.

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  • Anti-Neoplastic Effects of Calcium

    Striosomes Causal Changes Task Engagement

    Febuary, 2021

    Learning through affective responses, in which experiences are regarded as having a positive or negative valence (e.g., pleasure or fear, respectively), requires a judgment of the relative value of options. Several studies have linked a cluster of neurons in the subcortical basal ganglia, known as the striatum, to learning these valence-based responses.

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  • Anti-Neoplastic Effects of Calcium

    Mediating Palatability ­Guided Food and Water Consumption with Hindbrain Double­ Negative Feedback

    October, 2020

    Animal survival relies on neural mechanisms that trigger behaviors to meet basic bodily needs. Traditionally, motivated behaviors have been categorized into three phases.

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  • Anti-Neoplastic Effects of Calcium

    COVID­-19 Shows a Loss of Bcl­6­ Expressing T Follicular Helper Cells and Germinal Centers

    August, 2020

    A groundbreaking publication in the journal Cell suggests that in COVID-19 patients the differentiation of Tfh cells and formation of germinal centers within lymph nodes and spleen is inhibited. Molecules such as Bcl-6 were assessed using the TissueFAXS platform including the analysis applications StrataQuest and TissueQuest.

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  • Anti-Neoplastic Effects of Calcium

    Characterizing the Complex Microenvironment of Individual Immune Cells

    April, 2019

    Research groups from Yale University, USA published a paper in 'Cytometry' focusing on the phenotyping, quantification and visualization of immune cells and immune cell clusters within tissue sections using co-registration and IF multiplexed tissue cytometry. 

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  • Anti-Neoplastic Effects of Calcium

    Imaging of neural contributions to voluntary hand movement

    Febuary, 2019

    Skilled motor behavior requires the precise exchange of information between the muscles of the body and various levels of the central nervous system (CNS). Motor behavior, limb mechanics and neural control must be precisely coordinated in order to achieve the required movement.

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  • Anti-Neoplastic Effects of Calcium

    DNA-Binding of the Tet-Transactivator Curtails Antigen-Induced Lymphocyte Activation in Mice

    October, 2017

    Research groups from Austria, Australia, England and The Netherlands published a paper in 'Nature Communications' about the Tet-transactivator imparing the antigen-induced lymphocyte activation in mice.

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  • Anti-Neoplastic Effects of Calcium

    Tumor Specific Regulatory T Cells in the Bone Marrow of Breast Cancer Patients Selectively Upregulate the Emigration Receptor S1P1

    Febuary, 2017

    Groups from Germany (Heidelberg, Jena)  published a paper in the Journal 'Cancer Immunology, Immunotherapy'  regarding tumor-specifc regulatory T cells in the bone marrow of breast cancer patients.

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  • Anti-Neoplastic Effects of Calcium

    Quantitative Comparison and Reproducibility of Pathologist Scoring and Digital Image Analysis of Estrogen Receptor β2 Immunohistochemistry in Prostate Cancer

    July, 2016

    Research groups from USA, published a paper in 'Diagnostic Pathology' dealing with quantitative comparison and reproducibility of pathologist scoring and digital image analysis in prostate cancer.

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  • Anti-Neoplastic Effects of Calcium

    Anti-Neoplastic Effects of Calcium in Colorectal Cancer Potentiated by Active Vitamin D

    Febuary, 2015

    Groups from Vienna published a paper in 'The Journal of Steroid Biochemistry and Molecular Biology' about Vitamin D potentiating the anti-neoplastic effects of calcium in the colon.

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  • Researching the Role of Immune Checkpoints, IDO1 and PDL1, to Study Cutaneous Leishmaniasis

    In this interview we speak with Dr. Nidhi Dey about immune checkpoints, IDO1 and PDL1.

    What are IDO1 and PDL1, and how are they relevant to the study of cutaneous leishmaniasis?

    IDO1 and PDL1 are immune checkpoints that serve as regulators of the immune system. These checkpoints are relevant because they can suppress T-cell function. T-cells are a crucial component of the immune response against the parasite causing the disease.

    IDO1 and PDL1 can inhibit the activity of T-cells, which may hinder the body's ability to combat the parasite effectively. Understanding how these immune checkpoints function is essential for potentially developing therapies that target these checkpoints and enhance the immune response against the parasite.

    In a recent study conducted on patients with cutaneous leishmaniasis, it was observed that the transcripts of IDO1 and PDL1 were downregulated, meaning their expression was reduced upon treatment. This finding is significant because it suggests that treatment has the potential to reduce the inhibitory effects of IDO1 and PDL1 on T-cell function.

    As IDO1 and PDL1 suppress the immune response, their downregulation indicates an improved ability of the immune system to combat the parasite. This reduction in immune checkpoint expression is a positive outcome that may contribute to a more effective immune response and better treatment outcomes for patients.

    PDL1 can be used as a prognostic for treatment duration. Can you elaborate on how this information can impact patient care?

    The discovery that PDL1 expression serves as a prognostic marker for treatment duration has important implications for patient care. Patients with reduced PDL1 expression upon treatment were observed to respond to therapy more rapidly.

    This information can be utilized to assess the progress of treatment in individual patients. By monitoring PDL1 expression, healthcare providers can identify patients who are likely to recover faster.

    This data can be used to optimize treatment strategies, potentially reducing the duration of treatment, minimizing patient discomfort, and improving overall patient outcomes.

    What techniques were used in the study to analyze the spatial distribution of IDO1 and PDL1 in the skin tissue of patients with cutaneous leishmaniasis?

    The study employed several techniques to analyze the spatial distribution of IDO1 and PDL1 in the skin tissue of patients with cutaneous leishmaniasis. These techniques included low-resolution transcriptome profiling - NanoString DSP, spatial transriptomics using 10x Genomics Visium technology, whole slide imaging and single-cell image analysis with StrataQuest, TissueGnostics.

    These methods allowed researchers to examine the location and expression of IDO1 and PDL1 within the tissue samples. By using multiplexed imaging and transcriptome profiling, they could gain insights into how these immune checkpoints were distributed within the skin lesions.

    What meaning does neighborhood analysis of IDO1 and PDL1-positive cells have?

    During this study, it was found that among top cell types neighboring IDO+PDL1+ cells are CD8 memory T cells which indicates that IDO+PDL1+ cells signal to the neighboring T cells by reducing their T-cell function. Among other cell types regulatory T cells, dendritic cells and type 1 IFN macrophages were detected in the close vicinity.

    In comparison with other leishmaniasis models in India and Brazil, common genes were identified in correlation with IDO1 and PDL1. What are the implications of these common genes for future research or therapy development?

    Identifying common genes correlated with IDO1 and PDL1 across different leishmaniasis models suggests that these genes play a fundamental role in the disease, irrespective of the specific strain.

    The identified common genes may serve as targets for therapy applicable across various leishmaniasis strains. This could expedite the development of new treatments that are effective against a wide range of leishmaniasis variants, potentially improving therapeutic outcomes and broadening the scope of research for this infectious disease.

    Can you explain the concept of host-directed therapies in the context of cutaneous leishmaniasis and how IDO1 and PDL1 fit into this approach?

    Host-directed therapies involve targeting the host's immune response to enhance the outcome of an infectious disease. In the context of cutaneous leishmaniasis, IDO1 and PDL1 fit into this approach because they are immune checkpoints that inhibit T-cell function.

    By targeting these immune checkpoints with specific therapies such as immunotherapy or small molecule inhibitors, it is possible to boost the host's immune response against the parasite.

    This approach aims to reduce the suppression of T-cell function, enabling the immune system to mount a more effective defense against the parasite. Therefore, IDO1 and PDL1 represent potential targets for host-directed therapies in the treatment of cutaneous leishmaniasis.

    What potential treatment options, such as immunotherapy or small molecule inhibitors, are available for targeting IDO1 and PDL1 in the treatment of cutaneous leishmaniasis?

    In the treatment of cutaneous leishmaniasis, potential treatment options for targeting IDO1 and PDL1 include immunotherapy and small molecule inhibitors. PDL1 antibodies that are FDA-approved are already available in the market and are considered a form of immunotherapy.

    Additionally, small molecule inhibitors targeting IDO1 are in various phases of clinical trials. These therapies are designed to counteract the inhibitory effects of IDO1 and PDL1 on T-cell function, potentially enhancing the immune response against the parasite causing the disease.

    Utilizing these treatment options may lead to more effective and targeted approaches for managing cutaneous leishmaniasis.

    What was the role of CD68, a myeloid marker, in the analysis of IDO1 and PDL1 expression in skin tissue, and how does it relate to parasite burden?

    The parasite infects macrophages and CD68, a myeloid marker, was used in the study to identify myeloid cells in the skin tissue. Infected macrophages were found to express IDO1 and PDL1. The analysis, performed with StrataQuest image analysis software, revealed a positive correlation between the expression of IDO1 and PDL1 and the amastigote burden, which represents the parasite load.

    This suggests that macrophages, which express IDO1 and PDL1, are particularly affected by the parasite burden in cutaneous leishmaniasis, and as they were found to neighbor memory T cells, they are likely to reduce T-cell function which leads to parasite survival.  

    How might the study's findings on the role of immune checkpoints in cutaneous leishmaniasis impact clinical practice or the development of new therapies?

    The study's findings on the role of immune checkpoints, IDO1 and PDL1, in cutaneous leishmaniasis have several potential impacts on clinical practice and the development of new therapies. Understanding the significance of these immune checkpoints can lead to the development of targeted therapies that enhance the immune response and improve treatment outcomes.

    Clinically, the use of PDL1 as a prognostic marker for treatment duration can optimize treatment plans, reducing treatment duration and improving patient care. Overall, these findings advance our understanding of cutaneous leishmaniasis and offer promising avenues for the development of more effective therapies, potentially improving the management and outcomes of this infectious disease.

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  • The Role of Cytolytic CD8+ T Cells in Suppressing Ongoing HIV Replication in Spontaneous Controller Lymph Nodes

    Using TissueGnostics’ tissue cytometry solutions, a recent study revealed important information on how sustained cytolytic CD8+ T cells are central in determining long-term viral control in lymph nodes (LNs). This further elucidates the mechanisms behind the immune control over cancerous diseases, which may be used to develope treatments strategies for more effective results and better patient outcomes.

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