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  • MULTISPECTRAL CYTOMETERS

    TissueFAXS SPECTRA


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TissueFAXS SPECTRA 
Multispectral Tissue Cytometer

TissueFAXS SPECTRA systems provide Multispectral Imaging, Spectral Unmixing and Contextual Tissue Cytometry Image Analysis on the basis of the TissueFAXS platform.

SPECTRA technology is available on any TissueFAXS system configuration, be it upright, inverted, TissueFAXS Histo, TissueFAXS Fluo, TissueFAXS PLUS or Slide Loader-based (SL).

 

TissueFAXS SPECTRA
UNIQUE FEATURES

  • Multispectral fluorescence imaging
  • Spectral unmixing function
  • Brightfield imaging (optional)
  • 8 slides scanning
  • Slide ID scanner
  • Quantitative image analysis option

Technology

TissueFAXS SPECTRA is based on a liquid crystal tunable filter and the spectral unmixing engine of StrataQuest analysis software. Liquid crystal tunable filters are optical filters that use electronically controlled liquid crystal elements to transmit a selectable wavelength of light and exclude others. They can be tuned to specific wavelengths within the visible spectrum at high speeds. This makes them ideal for a quick building of the lambda stacks (a.k.a "Image Cubes" or "Spectral Cubes"), which then can be used for spectral unmixing.

This permits the separation of multiple colors in both, brightfield and fluorescence. The following graphic shows the process from building the lambda stack to color deconvolution via the StrataQuest spectral unmixing engine. The result is four greyscale channel images corresponding to the four unmixed colours and an overlay image analogous to a fluorescence overlay image.

 

 TF Spectra Technology

TissueFAXS Spectra



TissueFAXS SPECTRA SL

All imaging systems are modular and upgradable. Every system can be customized to offer the following capabilities: brightfield scanning, widefield fluorescence, confocal, multispectral as well as high-throughput. To exemplify TissueFAXS SPECTRA can be upgraded to the high-throughput TissueFAXS SPECTRA SL (image on the left side),

  • high-throughput scanning in multispectral fluorescence, widefield fluorescence as well as brightfield mode
  • fully automated loading/scanning of 120 slides at a time 
  • three 40 slide magazines with each magazine containing 20 two slide metal clips for a total of 120 standard slides.
  • alternatively 60 double sized slides
  • this system also supports TMA scanning

PROPERTIES

Design

Upright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Multispectral fluorescence, widefield fluorescence, brightfield scanning

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (standard configuration), 120 (slide loader configuration)

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

8

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048)
Color: CMOS Camera

Light Source

Solid-state light engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: Integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
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