CREATING THE RIGHT SPIRIT

TissueGnostics is an innovative enterprise on the cutting edge of science and technology our activities are guided by economic principles. We provide our customers with a new generation of imaging and analysis tools for basic and clinical research.

We offer objectivity, quality and innovation and continuously demand it from ourselves. Through const professional development, We maintain top-level scientific standards and competence by constantly reviewing and improving our processes, both scientific and business.

Hallmarks of our work include creativity, commitment and mutual understanding at all levels of operation. We make sure that our staff members enjoy a pleasant working environment that brings out their best performance.

EXCHANGING THOUGHTS

Everyday communication is about people exchanging ideas. We promote sincere communication in an atmosphere of trust and mutual respect to further creativity, satisfaction and social competence.

We are closely cooperating with clinicians and research facilities to ensure that our development work will always be customer-oriented. Custom requests and needs expressed by individuals are being incorporated to gear our products for a broad spectrum of challenges.

JOINING FORCES

We are making every effort to promote a sense of community enabling us to act as a team.

Our daily interactions are guided by mutual respect and pervaded by a peer-to-peer spirit. We strongly believe that social competence should not be a hollow phrase. Therefore we practice what we preach.

Our efforts are supported by solid training, continuous professional development, setting targets, and being responsible individuals. We cannot accept poor working conditions.

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b). To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

HISTORY

TissueGnostics was established in 2003 in Austria after nearly a decade of basic research. The goal was to supply scientists with qualitative and observer independent results for cell and tissue analysis in tissue context and this in automated format and significantly less time. Katja Österreicher, Georg Steiner and Dr. Rupert Ecker, the three co-founders of the company, with the aid of a dozen of development partners (Medical University Vienna, Memorial Sloan-Kettering Cancer Center New York, Ludwig Boltzmann Institute for Cancer Research, Vienna; Fred Hutchinson Cancer Center, Seattle; Brighamand Women`s Hospital, Boston; etc.) succeeded in reaching this target by 2005.

The product launch of TissueFAXS 1.0 was in 2006 and provided tissue imaging and automated identification and functional characterization of single cells in tissue sections. In contrast to morphometry, which provides values referring to the metric dimensions of cells, the term “tissue cytometry” refers to quantification of molecular parameters. Though methodically different, tissue cytometry exhibits a functional similarity to flow cytometry. While flow cytometer is restricted to cells in suspension (e.g. blood) and cannot be applied to solid tissue, tissue cytometry refers to the cytometric analysis (as opposed to morphometric analysis) of histological sections. TissueGnostics has been the first manufacturer of tissue cytometers offering a flow cytometry-like workflow (but applied to tissue sections).

Today in 2022 TissueGnostics is a successfull company with subsidiaries in the EU, the USA and China and with customers on all continents.