DNN
TissueGnostics’ new Deep Neural Network (DNN) was developed to handle nuclear segmentation in extremely dense tissue microenvironments with minimal user input. The shown example was challenging for nuclear segmentation due to the high cellular density, non-homogenous chromatin distribution pattern, and the weak DAPI (blue) signal intensity. The user was able to obtain a precise nuclear segmentation despite these three major issues using the DNN in StrataQuest 7.