TISSUE CYTOMETRY TECHNOLOGY

TG introduced Tissue Cytometry technology into image analysis software more than a decade ago with TissueQuest software in its first version. While, at a first look, it may appear to be just another form of displaying data, there is much more to it. Combined with segmentation images it is also a simple and intuitive tool to visually validate results. TG calls this „Backward Gating“ and „Forward Gating“.

Morphological filtering using Tissue Cytometry technology combined with appropriate planning of the analysis provides a highly flexible and capable instrument for extracting specific data from a sample (see image below).

The TissueFAXS Viewer
FREE TO DOWNLOAD

The TissueFAXS viewer is the freely downloadable and distributable viewer for viewing and annotating regions of interest in imaging projects scanned on TissueFAXS systems. The viewer is useful to both annotate objects of interest for, e.g., rescanning at higher magnifications, or for subsequent analysis with TissueGnostics analysis software, which imports the annotations as regions of interest.

Advanced StrataQuest

StrataQuest advanced features allow more experienced users to build their own solutions. The ideal tool to do this is StrataQuests logically structured Advanced Mode interface and highly adaptable sample viewer. Solutions created in Advanced Mode can be given an Easy Mode interface and saved as APPs for others to use. This capability, together with the possibility to plug in Matlab, ImageJ and FIJI solutions also make StrataQuest an ideal tool for imaging core facilities.

TISSUE CYTOMETRY TECHNOLOGY

TissueGnostics introduced Tissue Cytometry technology into image analysis software more than a decade ago. While, at a first look, it may appear to be just another form of displaying data, there is much more to it. Combined with segmentation images it is also a simple and intuitive tool to visually validate results. TG calls this "Backward Gating". Backward gating can be done for any measured parameter on histograms and scattergrams. The following examples demonstrate the versatility of backward gating. In the left image backward gating is used to visualise cells in which the HER2/neu staining has a closure angle of more than 180 degrees. In the right image backward gating is used on segmented nuclei positive for Ki-67. 

Morphological filtering using Tissue Cytometry technology combined with appropriate planning of the analysis provides a highly flexible and capable instrument for extracting specific data from a sample (see image below).

TissueFAXS Imaging Software 7.0 & 7.1

The new features of TissueFAXS 7.0 & 7.1 include improvements to the acquisition workflow, quality and visualiz ation of images, handling of TMAs, and the user interface. Here are some highlights:

• 7.1 NEW 3D VIEWER for Confocal imaging in TissueFAXS - via right click on a region and from the contextual menu select Region 3D View. Operation shortcuts enable an easy interaction the 3D image.

• 7.1 For TissueFAXS Spectra: Expanded spectral database for multispectral projects. Predefined markers have been added to make the unmixing process easier.

• 7.1 Improved Tissue Detection parameters/layout

• 7.1 Delete individual channels: TissueFAXS allows deleting channel images after regions were scanned.

• 7.1 Smart Focus Points strategy for focus points distribution uses the preview image to find the best positions in order to avoid low contrast areas, holes and areas outside the tissue plane.

• 7.0 The Region Viewer Toolbar has been streamlined for more easily navigating and editing images for visualization. Overlay changes can be made by checking or unchecking channels, and a new channel histogram has been added for easier signal detection. Lower and upper thresholds can be set more intuitively and any changes are made to the image in real time.

• 7.0 The new image compare sets function (Multi Region Viewer) allows to visually compare two or more acquired regions side by side.

• 7.0 comes with new visualization modes for acquired IF images including Pseudo IHC, providing a pathology view (pseudo coloured images) as well as the HDR-like mode (high dynamic range).

• 7.0 provides a dedicated database management section for a fast and easy search through all previously acquired projects.

• 7.0 Improved TMA block visualization and for the TF Spectra configuration, “spectral unmixing” is integrated.

• 7.0 allows for independent acquisition settings per slide within an experiment. Each individual slide can now contain its own independent acquisition settings for channels, focus, and camera settings within the same experiment.