TissueGnostics’ industry session at IUIS 2025 was a success, uniting immunologists around advances in spatial biology and AI-powered imaging. Thanks to Dr. Ecker, A/Prof. Mechtcheriakova, Prof. Batra, and all who joined us. Until next time!

Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin with multiplex immunostaining imaged with TissueFAXS Spectra, and StrataQuest for marker identification.

Multiplex fluorescence imaging of tonsil tissue analyzed in StrataQuest to quantify and map T-cell subsets within and around germinal centers.

This study highlights how contextual imaging with multiplexed immunofluorescence and automated analysis quantifies cell density and spatial distributions in ESCC. Results reveal MRPL13’s link to CD8+ T-cell infiltration in the tumor microenvironment.

Researchers combined spatial transcriptomics and multiplexed staining with TissueFAXS Spectra to identify a new CAF subset linked to stemness. StrataQuest enabled single-cell detection and tissue classification.

In HNSCC, CD8+ T-cell infiltration is often impaired. Chuwen Li et al. used mIF, TissueFAXS Spectra, and StrataQuest to image and analyze the spatial distribution of Gal9+ CAFs and CD8+ T cells, complementing prior spatial transcriptomics.

The newest StrataQuest supports in-depth neighborhood analysis with exportable data for statistics. It includes manifold learning (t-SNE, UMAP, SONG), violin plots, 3D diagrams, and other tools that give full control of data.

Dr. Qu Jiang (Leipzig) presents new findings on the oncogenic role of KDM1A in CLL. Using StrataQuest, her team quantified IF-stained murine spleen sections to assess leukemic burden after Kdm1a knockdown.

Through the power of spatial phenotyping, we gain the ability to shine a spotlight on different cell types and their environments.

High-throughput cytometers revolutionize tissue analysis by enabling rapid, automated whole-slide imaging and multiplex biomarker detection. With multispectral and confocal options, they provide deeper insights into cell phenotypes, tissue structures, and spatial interactions.

Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin, confirmed by multiplex immunostaining with TissueFAXS Spectra imaging platform, and StrataQuest image analysis software was employed for marker identification.

Dr. Andrea Papadopoulos and Trevor Khaba from Ndhlovu group, CESORA, Africa Health Research Institute, present their latest work on finding and characterizing new HIV reservoirs.

High-throughput tissue cytometry enables researchers to explore complex tissue microenvironments and discover novel biomarkers. With systems like TissueFAXS SL and StrataQuest analysis, large-scale scanning and advanced spatial analysis become efficient and precise.

StrataQuest contextual image analysis reveals how CD4+ T helper cells interact with their native tumor microenvironment in colorectal cancer, a leading malignancy in Europe.

StrataQuest software quantified NCAM and Ki67 distribution in kidney cancer samples, demonstrating how 2-plex IHC staining can be applied to spatial analysis of tumor markers.

Song et al. investigated a rectal cancer case with MSS/PD-L1-negative recurrent hepatopulmonary metastasis. Using TissueFAXS and StrataQuest, they characterized tumor-infiltrating immune cells, including CD4, CD8, CD163, PD1, and PD-L1.

Tumor growth depends on proximity to blood vessels. Using the IF Tumor Foci Angio App, researchers quantified tumor and non-tumor nuclei, vessel density, and distances of tumor cells from vasculature in brain tumor tissue, enabling advanced spatial analysis.

Heij et al. show that checkpoint molecule co-expression (PD-L1, TIGIT) in intrahepatic cholangiocarcinoma correlates with metastases and poor prognosis. TissueFAXS + StrataQuest enabled high-resolution mIF imaging and protein expression analysis.