Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin with multiplex immunostaining imaged with TissueFAXS Spectra, and StrataQuest for marker identification.

The evolving understanding of cancer biology underscores the pivotal role of epigenetic modifications - heritable yet reversible changes in gene expression that do not alter the DNA sequence.

This study highlights how contextual imaging with multiplexed immunofluorescence and automated analysis quantifies cell density and spatial distributions in ESCC. Results reveal MRPL13’s link to CD8+ T-cell infiltration in the tumor microenvironment.

Researchers from Soochow University investigated the B7-H4/USP2a axis in lung cancer using TissueFAXS Spectra for TMA imaging and StrataQuest for protein expression analysis.

Researchers combined spatial transcriptomics and multiplexed staining with TissueFAXS Spectra to identify a new CAF subset linked to stemness. StrataQuest enabled single-cell detection and tissue classification.

Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin, confirmed by multiplex immunostaining with TissueFAXS Spectra imaging platform, and StrataQuest image analysis software was employed for marker identification.

Felix Tsai, TG-AP Business Manager, shared an article featuring TLS as a key player in cancer and solutions to invesigate them.

StrataQuest contextual image analysis reveals how CD4+ T helper cells interact with their native tumor microenvironment in colorectal cancer, a leading malignancy in Europe.

A rare MSS-type CRC patient responded to immunotherapy. TissueFAXS SL and StrataQuest were used to image and quantify immune phenotypes.

Immunofluorescence image analysis is a powerful tool for profiling tumor microenvironments, but multiplexing and complex datasets bring major challenges. With tools like TissueFAXS SPECTRA and StrataQuest, researchers can optimize workflows and improve accuracy.

Multiplexing enables high-content phenotyping by analyzing multiple markers in a single tissue section. Combined with TissueFAXS CHROMA and StrataQuest Apps, it delivers detailed insights into tumor microenvironments and complex cellular interactions.

Tumor growth depends on proximity to blood vessels. Using the IF Tumor Foci Angio App, researchers quantified tumor and non-tumor nuclei, vessel density, and distances of tumor cells from vasculature in brain tumor tissue, enabling advanced spatial analysis.

Zhou et al. applied AI-assisted analysis to pancreatic adenocarcinoma, quantifying CD8+ TILs, cancer stem cells, and tumor budding. StrataQuest revealed immune and tumor features predictive of patient survival.

Imamura et al. characterized debrin+ tumor-infiltrating lymphocytes in lung cancer using StrataQuest for automated classification and spatial localization of immune phenotypes in tumor and stroma.

A study using TissueFAXS CHROMA and StrataQuest revealed that CD103+CD39+CD8+ TRM cells accumulate in colorectal liver metastases compared to adjacent liver tissue. These findings highlight their potential as therapeutic targets in advanced colorectal cancer.

Spatial phenotyping combines whole-slide imaging and advanced analysis to study cellular phenotypes in their native context. This approach is key in immunotherapy research, enabling insights into the tumor microenvironment and supporting informed treatment strategies.

Characterizing the tissue microenvironment reveals how cells and structures interact in health and disease. This blog highlights its role in cancer research and how TissueGnostics solutions enable advanced imaging and quantitative analysis.

Tissue microenvironment analysis reveals spatial relationships and cell interactions critical for understanding disease. This blog highlights its advantages, applications in melanoma research, and how TG solutions support high-quality analysis.