Dr. Uwe Ritter, LIT, reports on studies of Tregs in wound healing using 3D skin models and CD8+ T cells in tissue regeneration. He further describes the development of a co-cultured organoid detection pipeline published recently.

Wang et al. used spatial transcriptomics to characterize subsets of tumor-associated macrophages (TAMs) known as monocyte-derived TAMs. They identified a cluster of hypoxia-TAM oversecreting adrenomedullin with multiplex immunostaining imaged with TissueFAXS Spectra, and StrataQuest for marker identification.

Dr. Diana Mechtcheriakova, Medical University of Vienna, presents research on lymphoid structures in systemic immunity and anti-cancer responses, with applications in colorectal cancer.

A new study shows PD-1+ Treg/CTL ratio combined with TLS predicts prognosis and treatment response in gastric cancer using mIF, TissueFAXS, and StrataQuest.

A research group in China analyzed CD39+CD8+ T cells in esophageal squamous cell carcinoma. Based on mIF-stained samples acquired on the TissueFAXS platform, they propose these cells as new markers for diagnosis and prognosis.

The newest StrataQuest supports in-depth neighborhood analysis with exportable data for statistics. It includes manifold learning (t-SNE, UMAP, SONG), violin plots, 3D diagrams, and other tools that give full control of data.

Dr. Qu Jiang (Leipzig) presents new findings on the oncogenic role of KDM1A in CLL. Using StrataQuest, her team quantified IF-stained murine spleen sections to assess leukemic burden after Kdm1a knockdown.

Researchers investigated HHLA2 as a prognostic marker in LSCC using multiplexed IHC on TMA cores. TissueFAXS automated imaging and StrataQuest analysis revealed HHLA2 expression across immune cell populations.

Dr. Andrea Papadopoulos and Trevor Khaba from Ndhlovu group, CESORA, Africa Health Research Institute, present their latest work on finding and characterizing new HIV reservoirs.

StrataQuest contextual image analysis reveals how CD4+ T helper cells interact with their native tumor microenvironment in colorectal cancer, a leading malignancy in Europe.

A rare MSS-type CRC patient responded to immunotherapy. TissueFAXS SL and StrataQuest were used to image and quantify immune phenotypes.

Song et al. investigated a rectal cancer case with MSS/PD-L1-negative recurrent hepatopulmonary metastasis. Using TissueFAXS and StrataQuest, they characterized tumor-infiltrating immune cells, including CD4, CD8, CD163, PD1, and PD-L1.

Imamura et al. characterized debrin+ tumor-infiltrating lymphocytes in lung cancer using StrataQuest for automated classification and spatial localization of immune phenotypes in tumor and stroma.

Wang et al. investigated RNA and protein expression of immune checkpoint molecules in glioma, highlighting prognostic value. TissueFAXS SPECTRA enabled multispectral TMA scanning, and StrataQuest was used for in-depth phenotyping.

A study using TissueFAXS CHROMA and StrataQuest revealed that CD103+CD39+CD8+ TRM cells accumulate in colorectal liver metastases compared to adjacent liver tissue. These findings highlight their potential as therapeutic targets in advanced colorectal cancer.

Ingruber et al. examined pEMT-related proteins driving cisplatin resistance and recurrence in HNSCC. TissueFAXS and StrataQuest were applied for quantitative tissue cytometry and phenotyping.

Kaneko et al. (Scientific Reports, Nature) investigated CD4+ and CD8+ T-cell subsets in salivary glands of Sjögren’s syndrome patients using TissueFAXS with TissueQuest and StrataQuest for in-depth quantitative tissue cytometry.

Spatial phenotyping combines whole-slide imaging and advanced analysis to study cellular phenotypes in their native context. This approach is key in immunotherapy research, enabling insights into the tumor microenvironment and supporting informed treatment strategies.