RNA Scope+
Detect nuclei and quantify two RNA scope dot markers per cell in nucleus and/or cytoplasm for CISH and SISH assays, measuring up to 20 morphometric and intensity parameters plus per-cell dot count, area, and intensity metrics.
CD31, blood vessels, diameter
The RNA Scope+ App provides detection of nuclei based on appropriate staining as well as dot detection per cell within nucleus and/or cytoplasm for two dot markers in CISH and SISH experiments. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters. Dot parameters are provided per cell and include count, mean intensity, total dot area, and sum of intensity as well as area and intensity lists for all single dots
Melanie McCoy, The University of Western Australia
Original Image
Nuclei/cell detection
Red dot detection
Green dot detection
Application Note
01 Jun, 2023
Evaluating the Distance of Tumor Cells from Blood Vessels
Blog Post
17 May, 2023
An Intro to Deep Learning in Biomedical Imaging
We support the following file formats:
- TissueFAXS (aqproj)
- StrataFAXS II (vmic)
- PreciPoint (vmic, gtif)
- Generic BigTIFF Import
- Support for multipage BigTIFF files
- OME-TIFF
- JPEG, PNG, BMP, TIFF
- Zeiss (czi)
- Hamamatsu NanoZoomer (ndpi)
- Aperio (svs)
- Leica (scn)
- 3D HISTECH Pannoramic
- Mirax (mrxs)
- Olympus (vsi)
- More slide scanners to be added!
Related Apps
IHC Angio
Detect blood vessels based on appropriate stains (e.g. CD31), measure vessel and lumen areas, and export vessel number, density, and areas of vessels, endothelium, and lumina.
blood vessels, tumor vascularization, tumor microenvironment, brightfield
IF Tumor Vascularization
Segment tissue into tumor and stroma/healthy areas, detect CD31+ vessels, and quantify vessel number, area, density, and connectivity with configurable wall closing and distance linking.
vasculatization, cancer, stroma, tumor, blood vessels, CD31, spatial analysis, tumor microenvironment
Custom App development
Perfectly tailored image analysis solutions for your research.
You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.
