Chronic inflammatory diseases (CIDs) involve prolonged inflammation causing tissue damage and dysfunction.

Dr. Andrea Papadopoulos and Trevor Khaba from Ndhlovu group, CESORA, Africa Health Research Institute, present their latest work on finding and characterizing new HIV reservoirs.

The EU-funded SSBB project will train 15 early-stage researchers in drug development, infectiology, and immunology. TissueGnostics contributes as an industry partner to support preclinical testing and antibiotic tracking.

Dr. Nidhi S. Dey (University of York) presents her work on spatial analysis of IDO1 and PD-L1 in cutaneous leishmaniasis, uncovering immune checkpoint targets for treating this neglected parasitic disease.

Nadine Bayer, PhD (MedUni Vienna), presents her Leukemia (Nature) paper on microbial skin recolonization and immune response after allogeneic stem cell transfer.

Zambrano et al. characterized neutrophil extracellular traps (NETs) and NETotic cells in vaginal discharges of women with vaginitis using TissueFAXS i PLUS. The study highlights an underexplored aspect of public health.

In BMC Immunology, Mahlobo et al. analyzed T follicular regulatory cells (TFRs) in lymph nodes of HIV patients, focusing on their spatial localization, function, and heterogeneity. Quantification was performed using TissueFAXS i PLUS and TissueQuest.

This white paper details the first in-depth tissue cytometry analysis of CD3+ T-cell subsets in COVID-19 lungs. Using TissueFAXS PLUS and StrataQuest, researchers uncovered dysfunctional CD8+ T cells and expansion of cytotoxic CD4+ T cells driving impaired immunity.

Dr. Thomas Diefenbach and colleagues at the Ragon Institute analyzed B and T cell populations in COVID-19 patient lymph nodes using TissueFAXS and StrataQuest. Their microscopy and image analysis revealed disrupted germinal centers and impaired humoral immunity.

In Clinical Immunology, Kaneko et al. provide the first quantitative analysis of T-cell subset alterations in COVID-19 lungs and lymph nodes. Using TissueFAXS and StrataQuest, the team identified expansion of cytotoxic CD4+ T cells in severe cases.

A study in The Journal of Clinical Investigation (Mvaja et al.) shows the limited potential of duodenal CD8+ T cells in people living with HIV. High-content immunophenotyping was performed using TissueFAXS i PLUS and TissueQuest single-cell analysis.

PD-L1 expression has emerged as a predictor of treatment response in cutaneous leishmaniasis. Using StrataQuest image analysis, researchers quantified immune markers and parasites, highlighting PD-L1 as a potential host-directed therapeutic target.

Zhang et al. (Signal Transduction Targeted Therapy) studied Azvudine in SARS-CoV-2 infected macaques and COVID-19 patients. TissueFAXS Q and TissueQuest quantified viral load, immune status, and TUNEL staining in thymus tissue.

Collins et al. (Ragon Institute, Harvard/MIT/MGH) reveal key virologic and immunologic factors of durable HIV control. HIV+ lymph nodes were visualized via RNAScope and acquired using TissueFAXS Q (Cell Press).