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IHC 2

Separate two markers in IHC/HC slides, segment single cells into nucleus, perinuclear area, and/or cytoplasm, measure up to 20 intensity, statistic, and morphometric parameters per compartment.

single-cell analysis

cell culture

immunophenotyping

immunohistochemistry, phenotypes, biomarker, brightfield

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The IHC 2 App separates two markers (e.g. chromogen and counterstain) in an IHC or HC digital slide and segments single cells into nucleus, and/or perinuclear area and/or cytoplasm. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters which are displayed in scattergrams and histograms and can be exported.

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Original image

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Nuclei detection

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Phenotype detection

immunophenotyping

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Webinar

20 Jan, 2026

Decoding Metastatic Potential in Colorectal Cancer Using Tissue Cytometry

cell culture

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Webinar

19 Nov, 2024

Quantification of p-H2AX Foci in Co-cultured Cells Exposed to Radiation, Livia Sima

single-cell analysis

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White Paper

30 Mar, 2026

Understanding NeuroCOVID-19: SARS-CoV-2 Disrupts Astrocyte Homeostatic Functions

We support the following file formats:

  • TissueFAXS (aqproj)
  • StrataFAXS II (vmic)
  • PreciPoint (vmic, gtif)
  • Generic BigTIFF Import
  • Support for multipage BigTIFF files
  • OME-TIFF
  • JPEG, PNG, BMP, TIFF
  • Zeiss (czi)

  • Hamamatsu NanoZoomer (ndpi)
  • Aperio (svs)
  • Leica (scn)
  • 3D HISTECH Pannoramic
  • Mirax (mrxs)
  • Olympus (vsi)
  • More slide scanners to be added!

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Unmix three markers in IHC/HC slides, segment single cells into nucleus, perinuclear area, and/or cytoplasm, measure up to 20 intensity, statistic, and morphometric parameters per compartment.

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Image

Custom App development

Perfectly tailored image analysis solutions for your research.

You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.

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TissueGnostics GmbH
Taborstraße 10/2/8
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+43 1 216 11 90
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About Us

TissueGnostics provides advanced solutions for whole-slide imaging and image analysis in biological and clinical research. Our products help researchers to scan and analyze complex tissue samples, enabling more detailed insights into tissue structure, cellular interactions, and spatial cell landscape.

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