A new study shows PD-1+ Treg/CTL ratio combined with TLS predicts prognosis and treatment response in gastric cancer using mIF, TissueFAXS, and StrataQuest.

Researchers demonstrate TCL1A+ B cells drive TLS formation in OSCC and serve as a positive prognostic factor using mIF and TissueFAXS imaging.

Multiplex fluorescence imaging of tonsil tissue analyzed in StrataQuest to quantify and map T-cell subsets within and around germinal centers.

This study highlights how contextual imaging with multiplexed immunofluorescence and automated analysis quantifies cell density and spatial distributions in ESCC. Results reveal MRPL13’s link to CD8+ T-cell infiltration in the tumor microenvironment.

High-throughput cytometers revolutionize tissue analysis by enabling rapid, automated whole-slide imaging and multiplex biomarker detection. With multispectral and confocal options, they provide deeper insights into cell phenotypes, tissue structures, and spatial interactions.

Felix Tsai, TG-AP Business Manager, shared an article featuring TLS as a key player in cancer and solutions to invesigate them.

Immunofluorescence image analysis combined with tissue cytometry enables in-depth study of protein expression, cell interactions, and spatial biology. Learn how multiplex IF, whole-slide imaging, and AI-driven analysis are shaping the future of digital pathology.

Immunofluorescence image analysis is a powerful tool for profiling tumor microenvironments, but multiplexing and complex datasets bring major challenges. With tools like TissueFAXS SPECTRA and StrataQuest, researchers can optimize workflows and improve accuracy.

A new study shows that combining type I interferon (MxA) expression with CD8+ T cell density can predict patient response to neoadjuvant treatment in rectal adenocarcinoma. TissueFAXS CHROMA and StrataQuest enabled precise multispectral imaging and analysis.

Multiplexing enables simultaneous detection of multiple markers in a single tissue sample. By combining advanced staining, automated imaging, and AI-powered analysis, it provides deeper insights into the tumor microenvironment and precision medicine.

Multiplexing enables high-content phenotyping by analyzing multiple markers in a single tissue section. Combined with TissueFAXS CHROMA and StrataQuest Apps, it delivers detailed insights into tumor microenvironments and complex cellular interactions.

Researchers from Sichuan University performed transcriptomic and multiplex immunofluorescence analyses of glioma samples using TissueFAXS SPECTRA and StrataQuest. Findings highlight B7-H3 as a prognostic biomarker and potential immunotherapy target in glioma.

Heij et al. show that checkpoint molecule co-expression (PD-L1, TIGIT) in intrahepatic cholangiocarcinoma correlates with metastases and poor prognosis. TissueFAXS + StrataQuest enabled high-resolution mIF imaging and protein expression analysis.

Lin et al. investigated the UHRF1/DNMT1–MZF1 axis and its effect on CpG methylation of protease serine 3 isoforms in lung cancer. Using 7-marker multiplex staining with TissueFAXS SPECTRA and StrataQuest, they differentiated isoforms showing paradoxical effects.

StrataQuest enables high-content image analysis of IMC datasets. This case study demonstrates single-cell and proximity measurements across 30 markers within one tissue section for comprehensive phenotyping.

Dr. Thomas Diefenbach, The Ragon Institute Boston, presents various approaches for multiplexing IF, utilizing the power of TissueFAXS high-throughput automated slide scanning and StrataQuest powered high-end image analysis.

The IBEX protocol enables high-content multiplex imaging with hundreds of antibodies. When paired with StrataQuest, large IBEX datasets can be rapidly aligned, normalized, and analyzed for tissue classification, immunophenotyping, and spatial analysis.

Wang et al. (Molecular Therapy) applied multiplexing and TG’s cytometry solutions to study Tim-3 expression in tumor-infiltrating lymphocytes. Results support Tim-3 as a promising immune checkpoint target in cancer immunotherapy.