Quantitative Image-based Cytometry: Focus on the Foci!
Prof. Nicolas C. Hoch, University of Sao Paulo, is presenting his research on signaling and repair of DNA damage. He and his group are utilizing the power of TissueFAXS i PLUS slide scanning and the high-end image analysis solution, StrataQuest, for their research.
Webinar
Quantitative Image-based Cytometry: Focus on the Foci!
12 Oct, 2022
Webinar Summary
In this TG Academy webinar, Dr. Nicholas Hock (University of São Paulo) demonstrates how his lab applies quantitative image-based cytometry to study DNA damage signaling and repair. Using TissueFAXS and StrataQuest, he shows workflows for automated nuclear segmentation, cell cycle gating, and detection of nuclear foci such as γH2AX. The session highlights how image cytometry enables detailed single-cell analysis of DNA repair heterogeneity, cell cycle–dependent marker induction, and cytoplasmic foci quantification in viral studies.
Key Highlights
- DNA damage & cell cycle analysis: Quantitative imaging reveals heterogeneity in DNA repair responses linked to specific cell cycle stages.
- Nuclear foci detection: Advanced workflows allow quantification of DNA damage foci (e.g., 53BP1, γH2AX) at single-cell resolution.
- Cytoplasmic structures: Adapted strategies extend image
