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IF Leishmaniasis
Detect intracellular Leishmania in host cells, segment parasites, quantify parasites per cell, distinguish live/dead (specifc assay), and export 20 intensity, statistic, and morphometric parameters per compartment plus parasite count, mean intensityand size.
intracellular analysis
single-cell analysis
cell culture
macrophages, parasites, leishmaniasis, cell culture, protozoan parasites, fluorescence
The IF Leishmaniasis APP detects intracellular Leishmania parasites and segments them in the detected host cells. The number of parasites per cell is determined and living and dead parasites can be distinguished (live/dead assays). The APP outputs the following data: 20 intensity, statistic and morphometric parameters for each segmented cell compartment per marker. Number, mean intensity, sum of intensity, and size of parasites. Image courtasy: Prof Uwe Ritter, Institute of Immunology, University of Regensburg, Germany
Schmid M, Dufner B, Dürk J, et al. An Emerging Approach for Parallel Quantification of Intracellular Protozoan Parasites and Host Cell Characterization Using TissueFAXS Cytometry. PLoS One. 2015;10(10):e0139866. Published 2015 Oct 21. doi:10.1371/journal.pone.0139866
Image courtesy of Prof. Dr. Uwe Ritter LIT - Leibniz-Institut für Immuntherapie, Regensburg, Germany
Original image
Nuclei detection
Cell mask
Parasite detection
cell culture
Webinar
19 Nov, 2024
Quantification of p-H2AX Foci in Co-cultured Cells Exposed to Radiation, Livia Sima
single-cell analysis
White Paper
30 Mar, 2026
Understanding NeuroCOVID-19: SARS-CoV-2 Disrupts Astrocyte Homeostatic Functions
intracellular analysis
Application Note
14 Oct, 2024
Quantitative Analysis of Cultured Cells
We support the following file formats:
- TissueFAXS (aqproj)
- StrataFAXS II (vmic)
- PreciPoint (vmic, gtif)
- Generic BigTIFF Import
- Support for multipage BigTIFF files
- OME-TIFF
- JPEG, PNG, BMP, TIFF
- Zeiss (czi)
- Hamamatsu NanoZoomer (ndpi)
- Aperio (svs)
- Leica (scn)
- 3D HISTECH Pannoramic
- Mirax (mrxs)
- Olympus (vsi)
- More slide scanners to be added!
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Custom App development
Perfectly tailored image analysis solutions for your research.
You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.
