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IF Embryoid Bodies

Detect embryoid bodies/organoids in IF-stained samples, segment nuclei, identify cellular phenotypes in nuclei or membrane, and quantify organoid number, area, and phenotype distribution.

single-cell analysis

cell culture

organoid detection

embryoid bodies, cell culture

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The IF Embryoid Bodies App automatically detects embryoid bodies/organoids based on IF staining. It identifies nuclei based on DAPI staining or other nuclei dye and identifies additional phenotype markers in the nuclei/cell and/or membrane of the detected cells. It outputs number and area (µm2) of detected embryoid bodies/organoids, count of nuclei and count/% of cellular phenotypes.

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organoid detection

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Webinar

26 May, 2025

Exploring Immune Cell Interactions and Tissue Regeneration through Imaging

cell culture

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Webinar

19 Nov, 2024

Quantification of p-H2AX Foci in Co-cultured Cells Exposed to Radiation, Livia Sima

single-cell analysis

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White Paper

30 Mar, 2026

Understanding NeuroCOVID-19: SARS-CoV-2 Disrupts Astrocyte Homeostatic Functions

We support the following file formats:

  • TissueFAXS (aqproj)
  • StrataFAXS II (vmic)
  • PreciPoint (vmic, gtif)
  • Generic BigTIFF Import
  • Support for multipage BigTIFF files
  • OME-TIFF
  • JPEG, PNG, BMP, TIFF
  • Zeiss (czi)

  • Hamamatsu NanoZoomer (ndpi)
  • Aperio (svs)
  • Leica (scn)
  • 3D HISTECH Pannoramic
  • Mirax (mrxs)
  • Olympus (vsi)
  • More slide scanners to be added!

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IF Spheroids

Detect spheroids and nuclei-based cells, segment cellular compartments, quantify multiple IF markers and dot signals, and analyze spatial proximity of cell populations within spheroids.

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spheroid, membrane, 2D and 3D culture, Human colon cancer cell lines

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Organiod App

Detect cultured organoids using a machine learning classifier, quantify organoid number and total area (µm²), and classify organoids into defined size categories.

cell culture

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organoid quantification, immune cells, co-culture, machine learning

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Custom App development

Perfectly tailored image analysis solutions for your research.

You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.

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TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
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About Us

TissueGnostics provides advanced solutions for whole-slide imaging and image analysis in biological and clinical research. Our products help researchers to scan and analyze complex tissue samples, enabling more detailed insights into tissue structure, cellular interactions, and spatial cell landscape.

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