IF Cellular Contact
Determine cellular phenotypes in IF-stained samples, analyze direct cell–cell contacts, and quantify marker intensity, morphometric parameters, and number/% of contacting phenotypes.
cell contacts, spatial interactions, phenotype interactions, B cells, T cells, lung, lymph node, COVID-19, SARS-Cov-2
The IF Cellular Contact App allows to determine the cellular phenotype of specific IF stained cell populations and establishes the cellular contacts to their neighboring cells (the number of markers is technically unlimited). If needed, the App provides a separation of nuclei in tissues with high cellular densities. The App outputs parameters such as staining intensity per marker and morphometric parameters for each segmented cell/cell compartment as well as number and percentage of cells of different phenotypes in direct contact.
Kaneko N, Boucau J, Kuo HH, et al. Temporal changes in T cell subsets and expansion of cytotoxic CD4+ T cells in the lungs in severe COVID-19. Clin Immunol. 2022;237:108991. doi:10.1016/j.clim.2022.108991
Naoki Kaneko/Shiv Pillai (PI), Ragon Institute of MGH, MIT and Harvard, Boston, MA USA
Original Image
Nuclei detection
Phenotype 1 and Phenotype 2 detection
Phenotype 1 to 2 proximity 0-5μm
Phenotype 1 to 2 proximity 5-25μm
Phenotype 1 to 2 direct contact
Webinar
21 Oct, 2025
Multimodal Imaging of Cellular Senescence: Tissue Cytometry and Beyond
White Paper
17 Oct, 2025
Integrative Multiomics Approach Unveils Systemic Dysfunction in Colorectal Cancer (CRC)
White Paper
17 Oct, 2025
Integrative Multiomics Approach Unveils Systemic Dysfunction in Colorectal Cancer (CRC)
We support the following file formats:
- TissueFAXS (aqproj)
- StrataFAXS II (vmic)
- PreciPoint (vmic, gtif)
- Generic BigTIFF Import
- Support for multipage BigTIFF files
- OME-TIFF
- JPEG, PNG, BMP, TIFF
- Zeiss (czi)
- Hamamatsu NanoZoomer (ndpi)
- Aperio (svs)
- Leica (scn)
- 3D HISTECH Pannoramic
- Mirax (mrxs)
- Olympus (vsi)
- More slide scanners to be added!
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Custom App development
Perfectly tailored image analysis solutions for your research.
You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.
