IF Cardio Cell Culture Dots
Segment cultured cardiac cells, detect cardiomyocytes and fibroblasts, and quantify dot markers (CISH/FISH) per cell, including cell counts and dot number, area, and mean intensity.
cardiology, cardiomyocytes, cell culture, fibroblasts, troponin red, FISH
The IF Cardio Cell Culture Dot App provides cell segmentation, detection of cardiomyocytes (based an appropriate stain e.g. Troponin Red) and fibroblasts within cultured cardio cells plus one dot marker (CISH, FISH). The App outputs parameters such as number of cardiomyocytes, fibroblasts and dotpos cardiomyocytes, fibroblasts as well as number, area (μm²) and mean intensity of dots per cell.
Agatha Ribeiro da Silva, Prof. Jose E. Krieger (Heart Institute, University Soa Paulo)
Original Image
Nuclei detection
Dot detection
Cardiomyocytes (red), fibroblasts (green)
Application Note
14 Oct, 2024
Quantitative Analysis of Cultured Cells
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19 Jan, 2024
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We support the following file formats:
- TissueFAXS (aqproj)
- StrataFAXS II (vmic)
- PreciPoint (vmic, gtif)
- Generic BigTIFF Import
- Support for multipage BigTIFF files
- OME-TIFF
- JPEG, PNG, BMP, TIFF
- Zeiss (czi)
- Hamamatsu NanoZoomer (ndpi)
- Aperio (svs)
- Leica (scn)
- 3D HISTECH Pannoramic
- Mirax (mrxs)
- Olympus (vsi)
- More slide scanners to be added!
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Custom App development
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You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.
