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RNA Scope

Detect nuclei and single-marker dots (CISH/SISH), segment nucleus and/or cytoplasm, measure up to 20 intensity, statistic, and morphometric parameters, and quantify dot count, mean intensity, total area, intensity sum, and per-dot area/intensity per cell.

RNAScope, brightfield, CISH, ISH

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The RNA Scope App provides detection of nuclei based on appropriate staining as well as dot detection per cell within nucleus and/or cytoplasm for one dot marker in CISH and SISH experiments. Each segmented cell compartment is measured for up to 20 intensity, statistic and morphometric parameters. Dot parameters are provided per cell and include count, mean intensity, total dot area, and sum of intensity as well as area and intensity lists for all single dots.

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Original image

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Nuclei detection

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Nuclei and cell detection

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Nuclei/cell and dot detection

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Blog Post

15 Feb, 2023

Applications of AI in Cell Segmentation

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White Paper

17 Oct, 2025

Integrative Multiomics Approach Unveils Systemic Dysfunction in Colorectal Cancer (CRC)

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Application Note

14 Oct, 2024

Quantitative Analysis of Cultured Cells

We support the following file formats:

  • TissueFAXS (aqproj)
  • StrataFAXS II (vmic)
  • PreciPoint (vmic, gtif)
  • Generic BigTIFF Import
  • Support for multipage BigTIFF files
  • OME-TIFF
  • JPEG, PNG, BMP, TIFF
  • Zeiss (czi)

  • Hamamatsu NanoZoomer (ndpi)
  • Aperio (svs)
  • Leica (scn)
  • 3D HISTECH Pannoramic
  • Mirax (mrxs)
  • Olympus (vsi)
  • More slide scanners to be added!

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RNA Scope+

Detect nuclei and quantify two RNA scope dot markers per cell in nucleus and/or cytoplasm for CISH and SISH assays, measuring up to 20 morphometric and intensity parameters plus per-cell dot count, area, and intensity metrics.

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Custom App development

Perfectly tailored image analysis solutions for your research.

You have a specific research question that needs to be answered? We offer custom development of image analysis pipelines for specific tasks, be it detection of cellular phenotypes or quantification of tissue structures. After discussing your goals with one of our experts, you will get a ready-to-use App and be a step closer to an impactful publication.

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TissueGnostics GmbH
Taborstraße 10/2/8
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+43 1 216 11 90
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About Us

TissueGnostics provides advanced solutions for whole-slide imaging and image analysis in biological and clinical research. Our products help researchers to scan and analyze complex tissue samples, enabling more detailed insights into tissue structure, cellular interactions, and spatial cell landscape.

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