• RESEARCH POWERED BY TISSUEGNOSTICS

    White Papers


  • 1

White Papers

Please find a curated selection of research made possible by TissueGnostics software and systems below or in our searchable data base.

 

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

 IF_Tumor_Foci_Angio_overview-min.png

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

IF_Tumor_Foci_Angio_panel-min.png

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

IF_Tumor_Foci_Angio_scattergram.png

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies).
You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.