Checkpoint Molecules Predict Response to Immunotherapy in Cancer

FEATURED PUBLICATION

Checkpoint Molecules Predict Response to Immunotherapy in Cancer
cells

Heij et al. investigated the expression of checkpoint molecules in the tumor microenvironment of intrahepatic cholangio-carcinoma. They found that co-expression patterns of checkpoint molecules such as PD-L1 and TIGIT correlate with metastases and poor prognosis. TissueFAXS and StrataQuest were used to image the mIF-stained samples and assess protein expression.

Read the Publication
Community-developed Checklists Enhance Reproducibility of Microscopy Images

FEATURED PUBLICATION

Community-developed checklists for publishing images and image analyses
Nature Methods

QUAREP-LiMi is an initiative focused on promoting quality assessment and reproducibility for instruments and images in light microscopy. Dr. Rupert Ecker, CEO of TissueGnostics, participated in developing standardized guidelines for best practices in image communication. These checklists offer key recommendations on image formatting and annotation among others.

Read the Publication
AI-Assisted Analysis of ‘Anti-/Pro-Tumor’ Profiles for Pancreatic Adenocarcinoma

FEATURED PUBLICATION

AI-Assisted Analysis of ‘Anti-/Pro-Tumor’ Profiles for Pancreatic Adenocarcinoma
Cancer Biology & Medicine

To predict survival of patients with pancreatic adenocarcinoma, Zhou et al. performed an AI-assisted comprehensive analysis of CD8+ T cells, cancer stem cells (CSCs), and tumor budding (TB). They used StrataQuest image analysis software to automatically detect and quantify tumor-infiltrating CD8+ T cells, CD133+ CSCs, and CK19+ TB.

Read the Publication
Characterization of the Spatial Immune Context of Lung Cancer

FEATURED PUBLICATION

Characterization of the Spatial Immune Context of Lung Cancer
International Journal of Molecular Sciences

In a recent study, Imamura et al. investigated the role of debrin-expressing tumor infiltration lymphocytes (TIL) in lung cancer. The high-end image analysis solution, StrataQuest, was used for the automatic tissue classification and localization of CD3+/debrin+ cellular phenotypes in the tumor cell nest, as well as in the stroma.

Read the Publication
COL8A1 Promotes Tumor in Pancreatic Ductal Adenocarcinoma

FEATURED PUBLICATION

COL8A1 Promotes Tumor in Pancreatic Ductal Adenocarcinoma
Matrix Biology

Yan et al. investigated several collagen subtypes involved in pancreatic ductal adenocarcinoma desmoplasia. Based on this study, COL8A1 was suggested as a new marker for predicting poor chemotherapy responsiveness and survival. The TissueFAXS imaging platform and StrataQuest analysis solution were used to image and segment morphological structures.

Read the Publication
Erk1/2-Dependent HNSCC Cell Susceptibility to Erastin-Induced Ferroptosis

FEATURED PUBLICATION

Erk1/2-Dependent HNSCC Cell Susceptibility to Erastin-Induced Ferroptosis
cells

Savic et al. investigated the Erk 1/2 pathway role in ferroptosis induction by targeting xCT protein during the treatment of HNSCC. They found erastin to be a potent ferroptosis inducer which is strongly dependent on Erk 1/2 kinases. TissueFAXS and HistoQuest were used to image the IHC-stained samples and calculate the percentage of xCT-positive cells.

Read the Publication
Tumor Microenvironment Characterization of Rectal Cancer

FEATURED PUBLICATION

Case Report: Tumor Microenvironment Characterization of Rectal Cancer
Frontiers in Immunology

Song et al. published a case study investigating the genetics and tumor microenvironment of a rectal cancer patient with MSS/PD-L1-negative recurrent hepatopulmonary metastasis. TissueFAXS and StrataQuest were used to image stained tissue samples and to assess tumor infiltrating immune cells using, among others, CD4, CD8, CD163, PD1, and PD-L1 as markers.

Read the Publication
Adverse Effects of Single

FEATURED PUBLICATION

The impact of HIV infection on the frequencies, function, spatial localization and heterogeneity of T follicular regulatory cells (TFRs) within human lymph nodes
BMC Immunology

Mahlobo et al. published a study in BMC Immunology addressing the effect of HIV infections on frequencies, function, spatial localization and heterogeneity of T follicular regulatory cells (TFRs) in human lymph nodes. TFRs within lymph node tissues were quantified using TissueFAXS i PLUS and TissueQuest.

Read the Publication
Temporal changes

FEATURED PUBLICATION

Temporal changes in T cell subsets and expansion of cytotoxic CD4+ T cells in the lungs in severe COVID-19
Clinical Immunology

Kaneko et al. published a study in the Clinical Immunology journal focusing on the quantitative analysis of T-cell subset alterations in thoracic lymph nodes and lung samples of COVID-19 patients. With the help of TissueFAXS and StrataQuest, the authors were first to show the expansion of cytotoxic CD4+ T cells in the lungs of patients with severe COVID-19.

Read the Publication
Spatial Distribution

FEATURED PUBLICATION

Spatial distribution and functional analysis define the action pathway of Tim-3/Tim-3 ligands in tumor development
Molecular Therapy

In a recent publication in the journal Molecular Therapy, Wang et al (Cheeloo Medical College of Shandong University) combine multiplexing and TissueGnostics’ image cytometry solutions for the assessment of the expression level of Tim-3, a well-known immune checkpoint, in tumor infiltrating lymphocytes. This study nominates Tim-3 as a potential target for cancer immunotherapy.

Read the Publication
Multi-Antigen Imaging

FEATURED PUBLICATION

Multi-Antigen Imaging Reveals Inflammatory DC, ADAM17 and Neprilysin as Effectors in Keloid Formation
International Journal of Molecular Sciences

In a recent publication in the MDPI - International Journal of Molecular Sciences, Rath et al (University Hospital Heidelberg) combine multiplexing, TissueGnostics’ image cytometry and in-depth data mining to characterize the immune microenvironment including spatial relationships within keloid lesions. Using this approach, the authors were able to nominate potential therapeutic targets.

Read the Publication
SynNotch-CAR T

FEATURED PUBLICATION

SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma
Science Translational Medicine

A recent publication from the group of Choe et al. (University of California, UCSF) published in Science Translational research is exploring the therapeutic effectiveness of SynNotch-CAR T cells in a PDX glioblastoma mouse model. T cells in-situ were imaged/assessed by TissueFAXS and quantified via StrataQuest.

Read the Publication
CpG Methylation Influence on Protease Serine 3 Isoforms in Lung Cancer

FEATURED PUBLICATION

CpG Methylation Influence on Protease Serine 3 Isoforms in Lung Cancer
Acta Pharmaceutica Sinica B

Lin et al. investigated the UHRF1/DNMT1–MZF1 axis in the context of CpG methylation of protease serine 3, a protein with several isoforms that show paradoxical effects. To differentiate the isoforms, the researchers employed 7-marker multiplex staining and performed a quantitative analysis using TissueFAXS SPECTRA and StrataQuest.

Read the Publication

Filter publications

Customer Publications (A total of 3040 papers published)

3001
Regulation of matrix metalloproteinases and tissue inhibitor of metalloproteinases by cobalt chloride-stimulated hypoxia in primary and metastatic breast cancer cells

WY Lee, F Ou-Yang, MF Hou, SF Yang, SC Huang

European Journal of Cancer (Elsevier) , 10 July 2008

Oncology

3002
Biodistribution and kinetics of the novel selective oncolytic adenovirus M1 after systemic administration

X Huang, L Zhuang, Y Cao, Q Gao, Z Han, D Tang, H Xing, J Zhou, D Ma

European Journal of Cancer (Elsevier) , 10 July 2008

Oncology

3003
Melatonin Receptor (MT1) and Nestin Coexpression: an indicator of more advanced breast cancer?

O Rögelsperger, C Ekmekcioglu, M Svoboda, R Königsberg, M Klimpfinger, W Jäger, T Thalhammer

European Journal of Cancer (Elsevier) Austria, 10 July 2008

Oncology

3004
Insulin-like growth factor binding protein-3 (IGFBP-3) in the prostate and in prostate cancer: Local production, distribution and secretion pattern indicate a role in stromal-epithelial interaction

Petra Massoner, Petra Haag, Christof Seifarth, Andreas Jurgeit, Hermann Rogatsch, Wolfgang Doppler, Georg Bartsch, Helmut Klocker

The Prostate (Wiley) Austria, 05 May 2008

Oncology

Key words: IGFBP-3, prostate cancer, stromal-epithelial interaction, expression, secretion

3005
A standards based ontological approach to information handling for use by organizations providing human tissue for research

Mary E Edgerton, Carl Morrison, Virginia A LiVolsi, Christopher A Moskaluk, Stephen J Qualman, M Kay Washington, William E Grizzle

Cancer Informatics (SAGE Journals) USA, 10 April 2008

Biological Methods

3006
Acute anoxia stimulates proliferation in adult neural stem cells from the rat brain

Heinrich F Bürgers, Dominik W Schelshorn, Wolfgang Wagner, Wolfgang Kuschinsky, Martin H Maurer

Experimental Brain Research (Springer) Germany, 11 March 2008

Neuroscience

Key words: Stem cell marker, arythroid progenitor, ogd group, neural stem cell

3007
Lithium Promotes Adult Neural Progenitor Differentiation Via GSK3ß-Dependent Signaling Pathways

Heinrich F Bürgers, Dominik W Schelshorn, Wolfgang Kuschinsky and Martin H Maurer

The Open Enzyme Inhibition Journal () Germany, 02 January 2008

Cell Biology

Key words: Adult neural progenitor cell, Lithium, Two-dimensional gel electrophoresis, Rat.

3008
Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb

Martin H Maurer, Robert E Feldmann Jr, Heinrich F Bürgers, Wolfgang Kuschinsky

BMC Neuroscience (Springer) Germany, 01 January 2008

Neuroscience

Key words: Neural progenitor cells, neuroblasts, GFAP-positive cells

3009
Transplantation of adult neural progenitor cells transfected with vascular endothelial growth factor rescues grafted cells in the rat brain

Martin H Maurer, Christine Thomas, Heinrich F Bürgers, and Wolfgang Kuschinsky

International Journal of Biological Sciences (NCBI) Germany, 03 December 2007

Stem Cell Research

Key words: neural progenitor cell, neurosphere, transplantation, BrdU, Vascular Endothelial Growth Factor, VEGF.

3010
Automated recognition of cell phenotypes in histology images based on membrane- and nuclei-targeting biomarkers

Bilge Karaçali, Alexandra P Vamvakidou, Aydin Tözeren

BMC Medical Imaging (Springer) USA, 07 September 2007

Medical Image Analysis

Key words: Histology, biomarkers

3011
Chronische Inflammation als Promotor-und Behandlungstarget bei benigner Prostatahyperplasie (BPH) und beim Prostatakarzinom

G Kramer, D Mitteregger, A Maj-Hes, S Sevchenco, W Brozek

Der Urologe (Springer) Austria, 02 August 2007

Urology

3012
Induction of Autophagy Does Not Affect Human Rhinovirus Type 2 Production

Marianne Brabec-Zaruba, Ursula Berka, Dieter Blaas, Renate Fuchs

Journal of Virology (American Society for Microbiology) Austria, 01 August 2007

Virology

Key words: Rhynovirus

3013
Interactive Atlas of Histology: A Tool for Self-Directed Learning, Practice, and Self-Assessment

EZ Goubran, SP Vinjamury

Journal of Chiropractic Education () , 01 April 2007

Key words: atlases [publication type], chiropractic, education, histology, technology

3014
Is Benign Prostatic Hyperplasia (BPH) an Immune Inflammatory Disease?

Gero Kramer, Dieter Mitteregger, Michael Marberger

European Urology (Elsevier) Austria, 11 December 2006

Urology

Key words: Autoimmunity, Benign prostatic hyperplasia, Chronic inflammation, Cytokines, Immune response, Infection

Pathological State: Prostatic Hyperplasia

3015
Glycogen synthase kinase 3β (GSK3β) regulates differentiation and proliferation in neural stem cells from the rat subventricular zone

MH Maurer, JO Brömme, RE Feldmann, A Järve, F Sabouri, HF Bürgers, DW Schelshorn, C Krüger, A Schneider, W Kuschinsky

Journal of Proteome Research (ACS Publications) Germany, 03 November 2006

Cell Biology

Key words: Neural stem cell • Neurosphere • Glycogen Synthase Kinase 3â • Two-dimensional gel electrophoresis • Rat • Subventricular zone

3016
Microbubble-enhanced ultrasound to deliver an antisense oligodeoxynucleotide targeting the human androgen receptor into prostate tumours

Petra Haaga, Ferdinand Frauscherb, Johann Gradlb, Alexander Seitzb, Georg Schäferc, Jonathan R Lindnerd, Alexander L Klibanove, Georg Bartscha, Helmut Klockera, Iris E Eder

The Journal of Steroid Biochemistry and Molecular Biology (Elsevier) Austria, USA, 20 October 2006

Oncology

Key words: Antisense oligonucleotides, Androgen receptor, Microbubbles, Ultrasound, Prostate cancer

3017
ENGINEERING MAMMALIAN CELL TRANSCRIPTIONAL CONTROL SEQUENCES TO ACHIEVE IMPROVED TRANSCRIPTIONAL TARGETING OF PROSTATE TUMOURS

K Tieman, L Stanbridge, H Rippon, N Maitland

European Urology (Elsevier) , 16 September 2006

Oncology

Key words: Tumor, prostate

3018
EXPRESSION OF PBX1 AND UGT2B7 IN PROSTATE CANCER CELLS

V Berge, H Ramberg, T Eide, A Svindland, KA Taskén

European Urology (Elsevier) , 16 September 2006

Oncology

3019
MULTI-COLOUR STAINING AND TISSUEFAXS ANALYSIS FOR VALIDATION OF PROSTATE CANCER TISSUE MARKERS AND THERAPY TARGETS EXPRESSION

K Pfeil, C Seifarth, G Schäfer, G Steiner, R Rogojanu, G Gaillard, C Nowak, H Klocker

European Urology (Elsevier) , 16 September 2006

Oncology

3020
CASTRATION-INDUCED EPITHELIAL CELL DEATH IN PROSTATE TISSUE IS RELATED TO LOCALLY REDUCED IGF-1 LEVEL AND ACTION

P Wikström, N Ohlson, A Bergh, P Stattin

European Urology (Elsevier) , 16 September 2006

Urology

Key words: Prostate cancer

3021
3D parallel coordinate systems--a new data visualization method in the context of microscopy-based multicolor tissue cytometry

Streit M, Ecker RC, Osterreicher K, Steiner GE, Bischof H, Bangert C, Kopp T, Rogojanu R

Journal of the International Society for Advancement of Cytometry (Wiley) Austria, 05 May 2006

Cytometry

Key words: Polychromatic cells, automated microscopy-based multicolor tissue cytometry

3022
An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry

Ecker RC, Rogojanu R, Streit M, Oesterreicher K, Steiner GE

Journal of the International Society for Advancement of Cytometry (Wiley) Austria, 14 February 2006

Cytometry

Key words: Tissue citometry,

3023
Fast Automatic Segmentation of Nuclei in Microscopy Images of Tissue Sections

Laurain V, Ramoser H, Nowak C, Steiner GE, Ecker R

Engineering in Medicine and Biology Society (IEEEXplore) Austria, 18 January 2006

Medical Image Analysis

Key words: Nuclei, microscopy images, segmentation

3024
Leukocyte segmentation and classification in blood-smear images

Ramoser H, Adv Comput Vision GmbH, Wien, Laurain V, Bischof H, Ecker R

Engineering in Medicine and Biology Society (IEEEXplore) Austria, 18 January 2006

Hematology

3025
Systems biology and clinical cytomics: The 10th Leipziger workshop and the 3rd international workshop on slide-based cytometry

Attila Tárnok, Günther K Valet, Frank Emmrich

Cytometry Part A (Wiley) Germany, 20 December 2005

Cytometry

Key words: Predictive medicine, cytomics, biocomplexity, laser scanning cytometry, scanning fluorescence microscopy

3026
15th Annual Meeting of the German Society for Cytometry Deutsche Gesellschaft für Zytometrie (DGfZ) in collaboration with the Centre for Environmental Research (UFZ) Leipzig-Halle GmbH

Cell Proliferation (Wiley) Germany, 11 August 2005

Cytometry

3027
Cell Proliferation Volume 38, Issue 4, pages 204–214, August 2005

Cell Proliferation (Wiley) Germany, 11 August 2005

Cytometry

3028
Cell Proliferation Volume 38, Issue 4, pages 177–203, August 2005

Cell Proliferation (Wiley) Poland, 11 August 2005

Cytometry

3029
Welcome to the 15th Annual Meeting of the German Society for Cytometry, DGfZ

Cell Proliferation (Wiley) Germany, 11 August 2005

Cytometry

3030
Pimecrolimus leads to an apoptosis-induced depletion of T cells but not Langerhans cells in patients with atopic dermatitis

Wolfram Hoetzenecker, Rupert Ecker, Tamara Kopp, Anton Stuetz, Georg Stingl, Adelheid Elbe-Bürger

Journal of Allergy and Clinical Immunology (Elsevier) Austria, 06 April 2005

Allergy Research

Key words: Atopic dermatitis, Langerhans cells, T cells, apoptosis, corticosteroids, pimecrolimus

3031
Cytomics goes 3D: toward tissomics

Ecker RC, Tarnok A

Cytometry Part A (Wiley) Austria, 24 March 2005

Cytometry

3032
Regenerative and predictive medicine of cardiovascular disease: The 9th Leipziger workshop and the 2nd international workshop on slide based cytometry

Dominik Lenz, Markus J Barten, Sabine Hiller, Attila Tárnok, Ulrich Sack

Cytometry Part A (Wiley) Germany, 24 February 2005

Cytometry

Key words: Predictive medicine, regenerative medicine, stem cells, cytomics, laser scanning cytometry

3033
Autocrine IL-0 partially prevents differentiation of neonatal dendritic epidermal leukocytes into Langerhans cells

Souyet Chang-Rodriguez, Rupert Ecker, Georg Stingl, Adelheid Elbe-Bürger

Journal of Leukocyte Biology (JLB) Austria, 04 June 2004

Immunology

Key words: Antigen presentation/processing cytokines, cytokine receptors, dendritic cells skin

3034
Microscopy-based multicolor tissue cytometry at the single-cell level

Rupert C Ecker, Georg E Steiner

Cytometry Part A (Wiley) Austria, 07 May 2004

Cytometry

Key words: Cytomics, tissue cytometry, multicolor staining, quantitative histology, pattern recognition

3035
Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis.

Ecker RC, de Martin R, Steiner GE, Schmid JA

Journal of the International Society for Advancement of Cytometry (Wiley) Austria, 07 May 2004

Cytometry

3036
Decreased hippocampal mineralocorticoid:glucocorticoid receptor ratio is associated with low birth weight in female cynomolgus macaque neonates

Shirlene X Ong, Keefe Chng, Michael J Meaney, Jan P Buschdorf

Journal of Molecular Endocrinology (Society for Endocrinology) Austria, 06 April 2004

Reproductive Medicine

Key words: Glucocorticoid receptor cynomolgus macaque low birth weight MR:GR ratio

3037
Inhibition of restenosis by tissue factor pathway inhibitor: in vivo and in vitro evidence for suppressed monocyte chemoattraction and reduced gelatinolytic activity

Christoph W Kopp, Thomas Hölzenbein, Sabine Steiner, Rodrig Marculescu, Helga Bergmeister, Daniela Seidinger, Isabella Mosberger, Christoph Kaun, Manfred Cejna, Reinhard Horvat, Johann Wojta, Gerald Maurer, Bernd R Binder, Johannes M Breuss, Rupert C Ecker, Rainer de Martin, and Erich Minar

Blood (American Society of Hematology) Austria, 10 March 2004

Hematology

Key words: VSMC proliferation and migration, vascular restenosis

3038
Using web-based animations to teach histology

Marc AS Brisbourne, Susan S-L Chin, Erica Melnyk, David A Begg

The Anatomical Record (Wiley) Austria, 28 February 2002

Histology

Key words: Animation, computer-assisted learning, CAL, histology, Internet, education, medical, mental modeling, microscopy

3039
Detection of Epstein-Barr Virus in Hodgkin-Reed-Sternberg Cells: No Evidence for the Persistence of Integrated Viral Fragments in Latent Membrane Protein- (LMP-)-Negative Classical Hodgkin's Disease

Andrea Staratschek-Jox, Sascha Kotkowski, Gazanfer Belge, Thomas Rüdiger, Jörn Bullerdiek, Volker Diehl, Jürgen Wolf

The American Journal of Pathology (Elsevier) Germany, 10 October 2000

Virology

Key words: Hodgkin's disease, EBV genome, in situ hybridization

3040
Automated data acquisition by confocal laser scanning microscopy and image analysis of triple stained immunofluorescent leukocytes in tissue

Georg Erich Steiner, Rupert Christian Ecker, Gero Kramer, Felix Stockenhuber, Michael Johann Marberger

Journal of Immunological Methods (Elsevier) Austria, 07 March 2000

Medical Image Analysis

WANT TO KNOW MORE? CONTACT US

Blog update spatial
BLOG UPDATE
An Introduction to Spatial Tissue Cytometry

Cytometry encompasses several methods for investigating cells, such as their count, cell cycle state, phenotype, morphology, size etc. ...

NEWS
NEW Boxed Cytometer - Colubris

We have a lot of exciting plans to share for our big birthday, but we also have many important milestones to reflect on and remember...

conact bus
WANT TO KNOW MORE?
CONTACT US »
Festival Hacks
Subscribe to our scientific Infoletter
Subscribe to Newsletter
App Showcase
APP SHOWCASE
IHC IMMUNE SATUS IN SITU

The IHC Immune Status in Situ App uses the AI classifier to segment tissue into morphological entities such as tumor, stroma, and lymphocyte clusters. It further identifies single cells ...

Publications
TOOLS
Reference Publications

Explore our online database of reference publications to find tout how tissue cytometry can elevate your research

Appcenter
TOOLS
StrataQuest Appcenter

Explore our vast catalog of analysis solutions in our Strataquest Appcenter and get inspired by the variety of applications.

Nadine Bayer
TG ACADEMY WEBINAR
The Effect of Stem Cell Transplantation on Skin Microorganisms
Watch Webinar
Nadine Bayer, PhD
Medical University
of Vienna
Eurolab Article
ARTICLE
Spatial Tissue Cytometry: Success Stories - Eurolab Article

Read about tissue cytometry's potential in understanding the complexities of cell populations in the tissue environment

previous arrow
next arrow

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies).
You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.

Ok Decline
More information