• MULTISPECTRAL CYTOMETERS

    TissueFAXS CHROMA


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TissueFAXS CHROMA 
High-Speed Multispectral Tissue Cytometer

TissueFAXS CHROMA specializes in automated multispectral fluorescence whole slide scanning for up to 7 markers at a time made possible by an optimized filter set eliminating channel bleed through. This cost-effective high-speed slide scanner reaches its full potential when combined with single cell and contextual image analysis. This platform is a holistic solution for cellular phenotyping and in-depth tissue microenvironment characterization of IF processed tissue sections, TMAs, and cells on standard and oversized slides.

 

TissueFAXS CHROMA
KEY FEATURES

  • High resolution (16bit) multispectral fluorescence imaging
  • Simultaneous acquisition of up to 7 markers
  • Optimized for highest scanning speeds
  • In-depth cell/tissue microenvironment characterization
  • High-content phenotyping
  • Whole slide imaging
  • 8 slide capacity with continuous batch mode scanning
  • Supports scanning of tissue sections, biopsies, TMAs, fixed & cultured cells, and organoids
  • One click automation
  • Z stacking & extended focus for increased image quality
  • Quantitative image analysis supported by deep learning and machine learning

Technology

TissueFAXS CHROMA provides high-speed multispectral imaging and is made possible by a customized set of SpectraSplit filters. This specialized set of filters is optimized for a wide range of dyes with the appropriate excitation/emission spectra. This technology minimizes channel bleed-through passively and therefore represents a cost-effective alternative to the liquid crystal tuneable filter, which relies on lambda stacking and spectral unmixing of overlapping emission spectra. The acquisition of lambda stacks and spectral unmixing are time consuming and data-heavy processes. TissueFAXS CHROMA focuses on increasing sample throughput by minimizing multispectral scanning speeds without compromising spectral specificity and data integrity.

  • SpectraSplit DAPI
  • SpectraSplit 440 (Opal Polaris 480)
  • SpectraSplit 488 (AF488, FITC, Opal 520)
  • SpectraSplit Cy3 (AF555, Opal 570)
  • SpectraSplit 594 (AF594, TexasRed, Opal 620)
  • SpectraSplit 647 (AF647, Cy5, Opal 690)
  • SpectraSplit 750 (AF750, Cy7, Opal Polaris 780)

TissueFAXS CHROMA is an upright system with an 8 slide stage, high quality Zeiss objectives, state-of-the-art monochrome camera, high power illumination device, TissueFAXS scanning and management software.

 

TF Chroma Technology

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

 IF_Tumor_Foci_Angio_overview-min.png

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

IF_Tumor_Foci_Angio_panel-min.png

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

IF_Tumor_Foci_Angio_scattergram.png

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

PROPERTIES

Design

Upright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Multispectral fluorescence, widefield fluorescence

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (standard configuration)

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

7

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048)

Light Source

Solid-state broadband light engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest Fluo, StrataQuest Apps
single cell analysis - TissueQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: Integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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