• MULTISPECTRAL CYTOMETERS

    TissueFAXS CHROMA


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TissueFAXS CHROMA 
High-Speed Multispectral Tissue Cytometer

TissueFAXS CHROMA specializes in automated multispectral fluorescence whole slide scanning for up to 7 markers at a time made possible by an optimized filter set eliminating channel bleed through. This cost-effective high-speed slide scanner reaches its full potential when combined with single cell and contextual image analysis. This platform is a holistic solution for cellular phenotyping and in-depth tissue microenvironment characterization of IF processed tissue sections, TMAs, and cells on standard and oversized slides.

 

TissueFAXS CHROMA
KEY FEATURES

  • High resolution (16bit) multispectral fluorescence imaging
  • Simultaneous acquisition of up to 7 markers
  • Optimized for highest scanning speeds
  • In-depth cell/tissue microenvironment characterization
  • High-content phenotyping
  • Whole slide imaging
  • 8 slide capacity with continuous batch mode scanning
  • Supports scanning of tissue sections, biopsies, TMAs, fixed & cultured cells, and organoids
  • One click automation
  • Z stacking & extended focus for increased image quality
  • Quantitative image analysis supported by deep learning and machine learning

Technology

TissueFAXS CHROMA provides high-speed multispectral imaging and is made possible by a customized set of SpectraSplit filters. This specialized set of filters is optimized for a wide range of dyes with the appropriate excitation/emission spectra. This technology minimizes channel bleed-through passively and therefore represents a cost-effective alternative to the liquid crystal tuneable filter, which relies on lambda stacking and spectral unmixing of overlapping emission spectra. The acquisition of lambda stacks and spectral unmixing are time consuming and data-heavy processes. TissueFAXS CHROMA focuses on increasing sample throughput by minimizing multispectral scanning speeds without compromising spectral specificity and data integrity.

  • SpectraSplit DAPI
  • SpectraSplit 440 (Opal Polaris 480)
  • SpectraSplit 488 (AF488, FITC, Opal 520)
  • SpectraSplit Cy3 (AF555, Opal 570)
  • SpectraSplit 594 (AF594, TexasRed, Opal 620)
  • SpectraSplit 647 (AF647, Cy5, Opal 690)
  • SpectraSplit 750 (AF750, Cy7, Opal Polaris 780)

TissueFAXS CHROMA is an upright system with an 8 slide stage, high quality Zeiss objectives, state-of-the-art monochrome camera, high power illumination device, TissueFAXS scanning and management software.

 

TF Chroma Technology

IHC 2 APP: Ki-67 Nuclear Staining Analysis

The nuclear protein Ki67 is an established cell proliferation marker in research and clinics. Its staining and evaluation in tissue sections is an integral part of cancer diagnosis and prognosis.  Automated quantitative analysis of marker expression can provide a valuable supplement to visual evaluation. The IHC2 App is among the StrataQuest easiest-to-use analysis Apps and requires very little preparation time. This streamlined one-click analysis solution allows single cell detection, including the quantification of one additional marker within brightfield images.

The aim of the following case study was to assess (i) the total number of cells as well as (ii) the number and % of Ki-67positive cells. (a) shows colon tissue stained for hematoxylin (blue) to detect the nuclei and Ki67 (brown).

The first step of brightfield analysis is color separation. Using the color separation engine (b), the blue marker is automatically separated from the brown marker, as they are shown in grayscale images below.

The second step is the automatic identification of nuclei based on hematoxylin and Ki67 staining. Since Ki67 is located in the nuclei and is also covering the hematoxylin staining there, the nuclei can be detected from both markers. Analysis masks provide visual control of the correctness of the nuclear segmentation – here the nuclei are outlined in green (c).

After setting a cut-off on the intensity of the Ki67 marker in the corresponding scattergram, Ki-67 positive cells are shown with a red outline (d).

IF Dots original 95

Backward connection allows to check cells of interest separately on the corresponding scattergram (e), for example, for checking a possible outlier. The scattergram on the example is plotted for mean Ki-67 intensity vs nuclei size. The results can be exported in common CSV or Excel formats.

 

IF Dots min

Nuclear segmentation, as well as marker detection, is only one of the possibilities of the streamlined analysis solution available for StrataQuest. Get inspired by other analysis solutions in our StrataQuest App Center. Our team would also be glad to develop a personalized solution for you.

PROPERTIES

Design

Upright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Multispectral fluorescence, widefield fluorescence

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (standard configuration)

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

7

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048)

Light Source

Solid-state broadband light engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest Fluo, StrataQuest Apps
single cell analysis - TissueQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: Integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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