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High-Speed Multispectral Tissue Cytometer

TissueFAXS CHROMA specializes in automated multispectral fluorescence whole slide scanning for up to 7 markers at a time made possible by an optimized filter set eliminating channel bleed through. This cost-effective high-speed slide scanner reaches its full potential when combined with single cell and contextual image analysis. This platform is a holistic solution for cellular phenotyping and in-depth tissue microenvironment characterization of IF processed tissue sections, TMAs, and cells on standard and oversized slides.



  • High resolution (16bit) multispectral fluorescence imaging
  • Simultaneous acquisition of up to 7 markers
  • Optimized for highest scanning speeds
  • In-depth cell/tissue microenvironment characterization
  • High-content phenotyping
  • Whole slide imaging
  • 8 slide capacity with continuous batch mode scanning
  • Supports scanning of tissue sections, biopsies, TMAs, fixed & cultured cells, and organoids
  • One click automation
  • Z stacking & extended focus for increased image quality
  • Quantitative image analysis supported by deep learning and machine learning


TissueFAXS CHROMA provides high-speed multispectral imaging and is made possible by a customized set of SpectraSplit filters. This specialized set of filters is optimized for a wide range of dyes with the appropriate excitation/emission spectra. This technology minimizes channel bleed-through passively and therefore represents a cost-effective alternative to the liquid crystal tuneable filter, which relies on lambda stacking and spectral unmixing of overlapping emission spectra. The acquisition of lambda stacks and spectral unmixing are time consuming and data-heavy processes. TissueFAXS CHROMA focuses on increasing sample throughput by minimizing multispectral scanning speeds without compromising spectral specificity and data integrity.

  • SpectraSplit DAPI
  • SpectraSplit 440 (Opal Polaris 480)
  • SpectraSplit 488 (AF488, FITC, Opal 520)
  • SpectraSplit Cy3 (AF555, Opal 570)
  • SpectraSplit 594 (AF594, TexasRed, Opal 620)
  • SpectraSplit 647 (AF647, Cy5, Opal 690)
  • SpectraSplit 750 (AF750, Cy7, Opal Polaris 780)

TissueFAXS CHROMA is an upright system with an 8 slide stage, high quality Zeiss objectives, state-of-the-art monochrome camera, high power illumination device, TissueFAXS scanning and management software.


TF Chroma Technology

TMA Analysis with StrataQuest

Tissue microarrays (TMAs) have proven themselves as a standard molecular clinical diagnosis technique. TMAs are paraffin blocks in which up to 1000 individual tissue cores assembled in array fashion, allowing high-throughput histological analysis.

The TissueFAXS platform including the high-end image analysis solution StrataQuest provides specific workflow to scan and analyze the TMA samples containing multiple regions on one slide. TMA analysis in StrataQuest is available for images acquired in all imaging modalities (multispectral, fluorescence, brightfield).

In the first example, StrataQuest was utilized to analyze a TMA sample stained for hematoxylin (blue) and for E-cadherin present in the cell membranes of epithelial cells (brown). On the original image (a), E-cadherin is shown in brown and nuclei in blue. The aim was to detect the nuclei, the epithelial cells and the membranes of epithelial cells. The challenge with this analysis was that some cells do not contain nuclei.  

TMA Immune status Overview 15 name

Image (b) shows detected nuclei, covered by nuclei mask in light blue. Next only membranes were detected (c), which are shown in green; (d) shows the detection of the whole E-cadherin+ epithelial tissue, also marked in green.


Bild1 cut

In image (e) only cells with nuclei are identified, and the whole cell with the membrane is shown in blue, while the nuclei are covered by the light blue nuclei mask; (f) represents the nuclei in light blue and the total membranes in red.


Bild2 cut

Another example on TMA analysis is our IHC Immune Status in situ APP. Here the task was to develop a streamlined workflow for tissue classification (epithelium, stroma, lymphoid cluster).

Marker positive cells (for CD3+) were identified and spatial analysis was peformed, which would allow for the calculation of how many and which types of cells are located in defined distances from the object of interest (epithelium or lymphoid cluster). In the below example the App was applied to Tissue Microarrays (TMA) of an inflamed bladder.


TMA Immune status Overview 15 cut

One of the most notable features of StrataQuest is the machine learning-based tissue classification. To train the algorithm, the user marks regions assigned to different tissues and trains the classifier on the sample; later this workflow can be used on other samples. The following images represent the outcome of the classifier: (g) epithelium is detected in blue; (h) displays the lymphocyte cluster in light blue. These algorithms (or masks) can be combined to achieve a better visualization: (i) epithelium is displayed in blue, lymphocyte cluster in light blue and stroma in yellow.


TissueClassification cut

Beside the tissue classification itself, StrataQuest is capable of performing proximity analysis. It is possible to define specific proximity ranges; in the following case each range marked by different colors is 20µm. Nuclei detection was applied and CD3+ cells are shown in green, negative cells in yellow; (j) proximity ranges for the epithelium and (k) proximity ranges for the lymphocyte cluster.


TissueClassification2 cut

Apart from visual image analysis, StrataQuest outputs quantitative numbers. This way, it is possible to acquire and validate much more information. For example, StrataQuest can show how many cells are detected in each range: through the establishment of gates, 5.06% of CD3+ cells were found to be very close (in 20 µm range) to the epithelium.


scattergram Tumor Ranges edited cut

Further details about our various products and services can be found on our website. If you are interested in learning more about TissueGnostics solutions for TMA application or other tissue cytometry applications and our related services, please reach out to a member of the TissueGnostics team.



Upright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Multispectral fluorescence, widefield fluorescence

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (standard configuration)


Up to 7 Objectives (2.5-100x)




Monochrome: sCMOS camera (16-bit, 2048x2048)

Light Source

Solid-state broadband light engine

Tissue Microarray (TMA)


Image Analysis Software

contextual image analysis - StrataQuest Fluo, StrataQuest Apps
single cell analysis - TissueQuest

Supported Image Formats



TissueFAXS Viewer: Integrated, stand-alone freeware


TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
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