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TissueFAXS SL
HIGH-THROUGHPUT CYTOMETERS
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TissueFAXS SL
SLIDE LOADER

All TissueFAXS upright systems configurations (Fluo, Histo, PLUS) are also available as TissueFAXS SL (Slide Loader) high-throughput systems.

In this configuration, they are additionally equipped with an automatic slide loader and a specially adapted version of the TissueFAXS scanning and image management software.

This software version also includes TGs new SV (Slide Validator) technology.

STissueFAXS SL systems are also available in scan only configurations.

 

checkTissueFAXS SL systems can be upgraded to the same configurations as the non-loader systems (Histo and Fluo to PLUS, epifluorescence to confocal or high-speed).

TissueFAXS SL
KEY FEATURES

  • Fully automated high-throughput scanning - up to 120 slides
  • Option for oversized slides
  • Slide ID Scanner
  • Multi-channel fluorescence & brightfield
  • Extended Focus Imaging & Z-Stacking
  • Quantitative image analysis option
  • Image and metadata storage, management and archiving

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

 IF_Tumor_Foci_Angio_overview-min.png

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

IF_Tumor_Foci_Angio_panel-min.png

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

IF_Tumor_Foci_Angio_scattergram.png

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

CONFIGURATION

TissueFAXS SL systems are loaded with up to three 40 slide magazines with each magazine containing 20 two slide metal clips for a total of 120 standard slides.

Alternatively, 60 double sized (76 × 52 × 1 mm) slides can also be loaded and scanned, making TissueFAXS SL system one of the few in the market capable of doing this.

The clips are magnetically gripped and moved out to the stage for scanning. In this way, any contact of the slides with moving parts is prevented, safeguarding them against breakage.

TissueFAXS SL systems provide automatic scanning of brightfield and/or fluorescence slides. TMA scanning is supported in all configurations. Combined with TissueGnostics integrated high-speed cameras it provides high-throughput for slides with a speed and versatility in the top range.

TissueFAXS SL is capable of oil immersion scanning.

TissueFAXS SL Configuration

TissueFAXS SL
Workflow

TissueFAXS SL comes with a new version of the TissueFAXS software designed for the perfect handling of the high amount of samples, data validation and storage. There are two main workflows, a simple workflow for one type of slides and an advanced workflow for mixed samples types:

 

SL Flowchart

PROPERTIES

Design

Upright microscope, modular (Microscope, Automatic slide loader, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Fluorescence and brightfield scanning (upgrade to TissueFAXS SPECTRA SL or TissueFAXS Q SL)

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

120

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

9

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048px)
Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED
Solid-state Light Engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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