TissueFAXS SL
SLIDE LOADER

All TissueFAXS upright systems configurations (Fluo, Histo, PLUS) are also available as TissueFAXS SL (Slide Loader) high-throughput systems.

In this configuration, they are additionally equipped with an automatic slide loader and a specially adapted version of the TissueFAXS scanning and image management software.

This software version also includes TGs new SV (Slide Validator) technology.

TissueFAXS SL systems are also available in scan only configurations.

check TissueFAXS SL systems can be upgraded to the same configurations as the non-loader systems (Histo and Fluo to PLUS, epifluorescence to confocal or high-speed).

IF Dots App: Analysis of FISH

Fluorescence in situ hybridization (FISH) has been routinely used for decades in research and clinics to rapidly identify potential genetical changes within cells. This case study shows how TissueGnostics’ image analysis solution StrataQuest can be applied to evaluate FISH stainings. The most streamlined solution, the IF Dots App (runs within StrataQuest), can be applied to any analysis of dot-sized objects within the nuclei. Depending on the research needs, the following analysis can be combined with any other kind of analysis including AI-based tissue classification, phenotyping, spatial analysis, etc.

The aim of the following case study was to (i) identify individual nuclei and (ii) to assess how many FISH dots per individual nuclei are present.

The image below shows an overview of a triple staining: the nucleus stained by DAPI (blue), and two FISH markers, the housekeeping gene (green), and HER2/neu (red).

IF Dots original 95

The automated analysis starts with nuclei segmentation (a), where nuclei are identified by the intensity of the DAPI staining (nuclei are marked by the green outline). In the next step, the FISH dots are detected: in red the housekeeping gene in FITC channel (b) and in yellow for the HER2/neu in Acridine channel (c). Then these masks can be combined, as seen in (d).

IF Dots min

For further visualization of the analysis results, StrataQuest outputs the data in scattergrams. In the example below, the number of Acridine dots per nucleus (y-axis) is plotted against the FITC dots per nucleus (x-axis). The identified nuclei were gated for the condition “2 acridine dots per nuclei”, where the number of FITC dots can vary, and selected cells are shown in red contour via backward connection. Scattergrams showing the number of detected nuclei with each dot number combination are useful, especially with the Heat Map function enabled (bottom) which indicates those groups with a higher quantity of nuclei. To gain quantitative data for statistical analysis, the results can be immediately exported to Excel, CSV, and PDF formats.

IF Dots 2 min

FISH analysis is only one example of StrataQuest´s capabilities. If you are interested in how StrataQuest streamlined image analysis solution can enhance your research today, please reach out to a TissueGnostics team member for a demonstration.

Get to know more about FISH applications combined with TissueGnostics solutions in our blog posts:

CONFIGURATION

TissueFAXS SL systems are loaded with up to three 40 slide magazines with each magazine containing 20 two slide metal clips for a total of 120 standard slides.

Alternatively, 60 double sized (76 × 52 × 1 mm) slides can also be loaded and scanned, making TissueFAXS SL system one of the few in the market capable of doing this.

The clips are magnetically gripped and moved out to the stage for scanning. In this way, any contact of the slides with moving parts is prevented, safeguarding them against breakage.

TissueFAXS SL systems provide automatic scanning of brightfield and/or fluorescence slides. TMA scanning is supported in all configurations. Combined with TissueGnostics integrated high-speed cameras it provides high-throughput for slides with a speed and versatility in the top range.

TissueFAXS SL is capable of oil immersion scanning.