TissueFAXS PLUS

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TissueFAXS PLUS is an upright fluorescence and brightfileld system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with TissueQuest/HistoQuest or StrataQuest PLUS image analysis software.

STissueFAXS PLUS is also available in a scan only configuration.


checkTissueFAXS PLUS  can be upgraded to the TissueFAXS Q+ configuration and additionally equipped with fluorescence high-speed packages.


  • Fluorescence & brightfield scanning
  • Extended depth of focus
  • Compatible with oversized slides
  • One click-automation
  • Multi-channel fluorescence & brightfield scanning
  • Oil objectives
  • Quantitative image analysis
  • Live imaging option
  • Inverted configuration available
  • High-Speed fluorescence camera option
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TissueFAXS PLUS is the most versatile configuration of all TG upright systems.

The system comes equipped with an 8-slide stage. It can scan and analyse samples on slides and slide-based sample containers in both brightfield and epifluorescence modes. It can be equipped with supplementary components for contrast microscopy methods, like Differential interference contrast (DIC) microscopy in brightfield. It scans 10 channels in epifluorescence if the maximum of 10 single bandpass filters is fitted or more if multibandpass filters are used.

Objectives from all Zeiss objective classes with M24 thread can be fitted, the turret takes a maximum of seven.

TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. The TissueFAXS PLUS standard cameras are good examples of this successful strategy.

The right illumination is of paramount importance in light microscopy. TG provides fully system software-controlled solutions which are mostly maintenance free and have long lasting illuminantes.

The correct filters which guarantee pixel shift-free and low channel crosstalk are equally important to make fluorescence scanning easily accessible for all users of a TG system.

           TissueFAXS upright Cameras grey

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.


The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.


By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.


The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.



Upgright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Fluorescence and brightfield scanning

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (upgrade option to TissueFAXS PLUS SL for automated scanning of 120 slides)


Up to 7 Objectives (2.5-100x)




Monochrome: sCMOS camera (16-bit, 2048x2048px)
Color: CMOS camera (8-bit, 2048x2048px)

Light Source

Solid-state Light Engine

Tissue Microarray (TMA)


Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats



TissueFAXS Viewer: integrated, stand-alone Freeware


TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
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