Full 1
TissueFAXS PLUS
UPRIGHT BRIGHTFIELD & FLUORESCENCE CYTOMETERS
Full 1

TissueFAXS PLUS 
UPRIGHT BRIGHTFIELD AND FLUORESCENCE SYSTEM

TissueFAXS PLUS is an upright fluorescence and brightfileld system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with TissueQuest/HistoQuest or StrataQuest PLUS image analysis software.

STissueFAXS PLUS is also available in a scan only configuration.

 

checkTissueFAXS PLUS  can be upgraded to the TissueFAXS Q+ configuration and additionally equipped with fluorescence high-speed packages.

TissueFAXS PLUS
KEY FEATURES

  • Fluorescence & brightfield scanning
  • Extended depth of focus
  • Compatible with oversized slides
  • One click-automation
  • TMA & FISH/CISH
  • Multi-channel fluorescence & brightfield scanning
  • Oil objectives
  • Quantitative image analysis
  • Live imaging option
  • Inverted configuration available
  • High-Speed fluorescence camera option
  • Default
  • Title
  • Date
  • Random

CONFIGURATION

TissueFAXS PLUS is the most versatile configuration of all TG upright systems.

The system comes equipped with an 8-slide stage. It can scan and analyse samples on slides and slide-based sample containers in both brightfield and epifluorescence modes. It can be equipped with supplementary components for contrast microscopy methods, like Differential interference contrast (DIC) microscopy in brightfield. It scans 10 channels in epifluorescence if the maximum of 10 single bandpass filters is fitted or more if multibandpass filters are used.

Objectives from all Zeiss objective classes with M24 thread can be fitted, the turret takes a maximum of seven.

TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. The TissueFAXS PLUS standard cameras are good examples of this successful strategy.

The right illumination is of paramount importance in light microscopy. TG provides fully system software-controlled solutions which are mostly maintenance free and have long lasting illuminantes.

The correct filters which guarantee pixel shift-free and low channel crosstalk are equally important to make fluorescence scanning easily accessible for all users of a TG system.

           TissueFAXS upright Cameras grey

Leishmaniasis parasite detection in cell culture and tissue: Leishmaniasis APP

Leishmania donovani parasites are the causative agents of zoonotic and anthroponotic visceral leishmaniasis. There is no prophylactic vaccine and available drugs are either expensive and/or show significant toxicity, making the research on leishmania biology essential for a detailed understanding of the dispersal of these obligatory-intracellular parasites and their host phagocytes in the skin [1].

The Leishmaniasis APP within TissueGnostics contextual image analysis software, StrataQuest, was designed to assist in immunology research into Leishmaniasis and can be adapted for research on other intracellular parasites. It can be used both on cell cultures and tissue sections. Its main function is to automatically detect parasite stages in cell compartments and quantify them in context with specific immune markers. In this case study, we will show in detail how simple and straightforward the analysis is.

The aim of this project was to detect how many cells are infected by leishmaniasis and the number of leishmaniasis parasites per cell.

The image below shows a mouse skin-draining lymph node stained with DAPI (nucleus, blue).

 IF_Tumor_Foci_Angio_overview-min.png

Nuclei detection based on DAPI staining is one of the most crucial first steps in image analysis; (a) here nuclei are outlined in green. Starting from this, the software defines the whole cell around the nuclei, outlined in yellow for representation (b); (c) detection of parasites inside the cell as shown here as pink dots; (d) combined masks showing cells in yellow and Leishmaniasis parasites in pink.

IF_Tumor_Foci_Angio_panel-min.png

The obtained data is visualized within StrataQuest using scattergrams. In the example below, (e) cells containing parasites are highlighted in red by selecting them in the proper scattergram (in this case, parasites count vs cell size). The individual cells within an image can be chosen and their exact position in the corresponding scattergram is shown – this is a feature available in StrataQuest called forward connection, especially useful for validation of the selected gates.

IF_Tumor_Foci_Angio_scattergram.png

In the end, all results with many parameters to choose from can be outputted into common file formats (Excel, PDF, CSV) for further statistical analysis.

However, the power of StrataQuest´s App-based streamlined workflow does not end with cell/parasite detection. For further deciphering of Leishmania life cycle aspects and distribution processes, the parasite load and the status of parasite stages, e.g. “live/dead” can also be evaluated to obtain additive information. The IF Dots APP enables the analysis of FISH-stained leishmaniasis parasites.

A recent paper utilizing StrataQuest´s analysis software showed that programmed death-ligand 1 (PD-L1) expression is predictive of clinical response to treatment in patients with leishmaniasis [2]. If you are interested in performing detailed context-based quantitative analysis, explore the potential of StrataQuest and StrataQuest Apps.

 

Sources:

  1. Doehl JSP, Ashwin H, Brown N, Romano A, Carmichael S, Pitchford JW, Kaye PM. Spatial Point Pattern Analysis Identifies Mechanisms Shaping the Skin Parasite Landscape in Leishmania donovani Infection. Front Immunol. 2021 Dec 16;12:795554. doi: 10.3389/fimmu.2021.795554.
  2. Nidhi S. Dey, Paul M. Kaye, Shalindra Ranasinghe. Early reduction in PD-L1 expression predicts faster treatment response in human cutaneous leishmaniasis. October 5, 2021. J Clin Invest. 2021;131(22):e142765. doi.org/10.1172/JCI142765.
  3. TissueGnostics, 2022. White paper. PD-L1 raises new hope in the treatment of cutaneous leishmaniasis.

PROPERTIES

Design

Upgright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Fluorescence and brightfield scanning

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (upgrade option to TissueFAXS PLUS SL for automated scanning of 120 slides)

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

9

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048px)
Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED
Solid-state Light Engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone Freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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