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TissueFAXS PLUS
UPRIGHT BRIGHTFIELD & FLUORESCENCE CYTOMETERS
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TissueFAXS PLUS 
UPRIGHT BRIGHTFIELD AND FLUORESCENCE SYSTEM

TissueFAXS PLUS is an upright fluorescence and brightfileld system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with TissueQuest/HistoQuest or StrataQuest PLUS image analysis software.

STissueFAXS PLUS is also available in a scan only configuration.

 

checkTissueFAXS PLUS  can be upgraded to the TissueFAXS Q+ configuration and additionally equipped with fluorescence high-speed packages.

TissueFAXS PLUS
KEY FEATURES

  • Fluorescence & brightfield scanning
  • Extended depth of focus
  • Compatible with oversized slides
  • One click-automation
  • TMA & FISH/CISH
  • Multi-channel fluorescence & brightfield scanning
  • Oil objectives
  • Quantitative image analysis
  • Live imaging option
  • Inverted configuration available
  • High-Speed fluorescence camera option
  • Default
  • Title
  • Date
  • Random

CONFIGURATION

TissueFAXS PLUS is the most versatile configuration of all TG upright systems.

The system comes equipped with an 8-slide stage. It can scan and analyse samples on slides and slide-based sample containers in both brightfield and epifluorescence modes. It can be equipped with supplementary components for contrast microscopy methods, like Differential interference contrast (DIC) microscopy in brightfield. It scans 10 channels in epifluorescence if the maximum of 10 single bandpass filters is fitted or more if multibandpass filters are used.

Objectives from all Zeiss objective classes with M24 thread can be fitted, the turret takes a maximum of seven.

TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. The TissueFAXS PLUS standard cameras are good examples of this successful strategy.

The right illumination is of paramount importance in light microscopy. TG provides fully system software-controlled solutions which are mostly maintenance free and have long lasting illuminantes.

The correct filters which guarantee pixel shift-free and low channel crosstalk are equally important to make fluorescence scanning easily accessible for all users of a TG system.

           TissueFAXS upright Cameras grey

IHC 2 APP: Ki-67 Nuclear Staining Analysis

The nuclear protein Ki-67 is an established cell proliferation marker in research and clinics. Its staining and evaluation in tissue sections is an integral part of cancer diagnosis and prognosis.  Automated quantitative analysis of marker expression can provide a valuable supplement to visual evaluation. The IHC2 App is among the StrataQuest easiest-to-use analysis Apps and requires very little preparation time. This streamlined one-click analysis solution allows single cell detection, including the quantification of one additional marker within brightfield images.

The aim of the following case study was to assess (i) the total number of cells as well as (ii) the number and % of Ki-67 positive cells. (a) shows colon tissue stained for hematoxylin (blue) to detect the nuclei and Ki-67 (brown).

The first step of brightfield analysis is color separation. Using the color separation engine (b), the blue marker is automatically separated from the brown marker, as they are shown in grayscale images below.

The second step is the automatic identification of nuclei based on hematoxylin and Ki-67 staining. Since Ki-67 is located in the nuclei and is also covering the hematoxylin staining there, the nuclei can be detected from both markers. Analysis masks provide visual control of the correctness of the nuclear segmentation – here the nuclei are outlined in green (c).

After setting a cut-off on the intensity of the Ki-67 marker in the corresponding scattergram, Ki-67 positive cells are shown with a red outline (d).

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Backward connection allows to check cells of interest separately on the corresponding scattergram (e), for example, for checking a possible outlier. The scattergram on the example is plotted for mean Ki-67 intensity vs nuclei size. The results can be exported in common CSV or Excel formats.

 

IF Dots min

Nuclear segmentation, as well as marker detection, is only one of the possibilities of the streamlined analysis solution available for StrataQuest. Get inspired by other analysis solutions in our StrataQuest App Center. Our team would also be glad to develop a personalized solution for you.

PROPERTIES

Design

Upgright microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Fluorescence and brightfield scanning

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (upgrade option to TissueFAXS PLUS SL for automated scanning of 120 slides)

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

9

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048px)
Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED
Solid-state Light Engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone Freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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