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TISSUE & CELL CULTURE CYTOMETERS
TissueFAXS i PLUS
BRIGHTFIELD & FLUORESCENCE IMAGING
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TissueFAXS i PLUS 
INVERTED BRIGHTFIELD AND FLUORESCENCE SYSTEM

TissueFAXS i PLUS is an inverted fluorescence and brightfield system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with TissueQuest/HistoQuest or StrataQuest PLUS image analysis software.

SThe TissueFAXS i PLUS is also available in a scan only configuration.

 

checkThe TissueFAXS i PLUS can be upgraded to the TissueFAXS iQ+ configuration and additionally equipped with fluorescence high-speed packages.

TissueFAXS i PLUS
KEY FEATURES

  • Multi-channel fluorescence & brightfield
  • 8 slides, 1 micro well plate, 1 petri dish
  • Automatic tissue detection
  • Time lapse scanning, live imaging
  • Extended focus, stitching & illumination correction
  • Quantitative image analysis
  • TMA & FISH/CISH scanning & analysis
  • Image and metadata storage, management and archiving
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  • Date
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CONFIGURATION

TissueFAXS i PLUS is the most versatile configuration of all TG inverse systems. It can scan and analyse samples on slides, in microtiter plates and in Petri dishes in both brightfield and epifluorescence modes. It can be equipped with supplementary components for contrast microscopy methods, like Differential interference contrast (DIC) microscopy in brightfield. It scans 5 channels in epifluorescence if the maximum of 5 single bandpass filters is fitted or more if multibandpass filters are used.

The system comes equipped with an 8-slide stage which can also take a microtiter plate insert as an alternative. Inserts for Petri dishes and customized inserts for other sample containers are available on demand. A four slide/one microtiter plate stage is also available.

TissueFAXS i PLUS supports Live Imaging, as do all other inverse stand-based configurations. CO2, O2 and temperature control modules are available, as is the choice of small stage top incubators or conventional incubation enclosures. The environment controls are not integrated into the TissueFAXS control software (being usually set for longer time periods) and can be controlled via the microscopes touch screen. The TissueFAXS control software provides time lapse scanning.

Objectives from all Zeiss objective classes with M24 thread can be fitted, the turret takes a maximum of 6. TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. The TissueFAXS i PLUS standard cameras are good examples of this successful strategy. The right illumination is of paramount importance in light microscopy. TG provides fully system software-controlled solutions which are mostly maintenance free and have long lasting illuminantes. The correct filters which guarantee pixel shift-free and low channel crosstalk are equally important to make fluorescence scanning easily accessible for all users of a TG system.

TissueFAXS Scanning and Management software integrates all the hardware components into an easy to use workflow with full, walk-away automation capabilities.



TissueFAXS inverted Configuration

TissueFAXS iPlus LiveImaging

IF Dots App: Analysis of FISH

Fluorescence in situ hybridization (FISH) has been routinely used for decades in research and clinics to rapidly identify potential genetical changes within cells. This case study shows how TissueGnostics’ image analysis solution StrataQuest can be applied to evaluate FISH stainings.  The most streamlined solution, the IF Dots App (runs within StrataQuest), can be applied to any analysis of dot-sized objects within the nuclei. Depending on the research needs, the following analysis can be combined with any other kind of analysis including AI-based tissue classification, phenotyping, spatial analysis, etc.

The aim of the following case study was to (i) identify individual nuclei and (ii) to assess how many FISH dots per individual nuclei are present.

The image below shows an overview of a triple staining: the nucleus stained by DAPI (blue), and two FISH markers, the housekeeping gene (green), and HER2/neu (red).

IF Dots original 95

The automated analysis starts with nuclei segmentation (a), where nuclei are identified by the intensity of the DAPI staining (nuclei are marked by the green outline). In the next step, the FISH dots are detected: in red the housekeeping gene in FITC channel (b) and in yellow for the HER2/neu in Acridine channel (c). Then these masks can be combined, as seen in (d).

 

IF Dots min

For further visualization of the analysis results, StrataQuest outputs the data in scattergrams. In the example below, the number of Acridine dots per nucleus (y-axis) is plotted against the FITC dots per nucleus (x-axis). The identified nuclei were gated for the condition “2 acridine dots per nuclei”, where the number of FITC dots can vary, and selected cells are shown in red contour via backward connection. Scattergrams showing the number of detected nuclei with each dot number combination are useful, especially with the Heat Map function enabled (bottom) which indicates those groups with a higher quantity of nuclei. To gain quantitative data for statistical analysis, the results can be immediately exported to Excel, CSV, and PDF formats.

 

IF Dots 2 min

FISH analysis is only one example of StrataQuest´s capabilities. If you are interested in how StrataQuest streamlined image analysis solution can enhance your research today, please reach out to a TissueGnostics team member for a demonstration.

Get to know more about FISH applications combined with TissueGnostics solutions in our blog posts:

PROPERTIES

Design

Inverted microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Fluorescence and brightfield scanning

Compatible Slide Formats

Standard Slides, Over-sized Slides, Petri Dishes, Well-Plates

Slide Capacity

8

Objectives

Up to 6 Objectives (2.5-100x)

FL-Channels

5

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048px)
Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED
Solid-state Light Engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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