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TISSUE & CELL CULTURE CYTOMETERS
TissueFAXS i PLUS
BRIGHTFIELD & FLUORESCENCE IMAGING
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TissueFAXS i PLUS 
INVERTED BRIGHTFIELD AND FLUORESCENCE SYSTEM

TissueFAXS i PLUS is an inverted fluorescence and brightfield system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with TissueQuest/HistoQuest or StrataQuest PLUS image analysis software.

SThe TissueFAXS i PLUS is also available in a scan only configuration.

 

checkThe TissueFAXS i PLUS can be upgraded to the TissueFAXS iQ+ configuration and additionally equipped with fluorescence high-speed packages.

TissueFAXS i PLUS
KEY FEATURES

  • Multi-channel fluorescence & brightfield
  • 8 slides, 1 micro well plate, 1 petri dish
  • Automatic tissue detection
  • Time lapse scanning, live imaging
  • Extended focus, stitching & illumination correction
  • Quantitative image analysis
  • TMA & FISH/CISH scanning & analysis
  • Image and metadata storage, management and archiving
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CONFIGURATION

TissueFAXS i PLUS is the most versatile configuration of all TG inverse systems. It can scan and analyse samples on slides, in microtiter plates and in Petri dishes in both brightfield and epifluorescence modes. It can be equipped with supplementary components for contrast microscopy methods, like Differential interference contrast (DIC) microscopy in brightfield. It scans 5 channels in epifluorescence if the maximum of 5 single bandpass filters is fitted or more if multibandpass filters are used.

The system comes equipped with an 8-slide stage which can also take a microtiter plate insert as an alternative. Inserts for Petri dishes and customized inserts for other sample containers are available on demand. A four slide/one microtiter plate stage is also available.

TissueFAXS i PLUS supports Live Imaging, as do all other inverse stand-based configurations. CO2, O2 and temperature control modules are available, as is the choice of small stage top incubators or conventional incubation enclosures. The environment controls are not integrated into the TissueFAXS control software (being usually set for longer time periods) and can be controlled via the microscopes touch screen. The TissueFAXS control software provides time lapse scanning.

Objectives from all Zeiss objective classes with M24 thread can be fitted, the turret takes a maximum of 6. TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. The TissueFAXS i PLUS standard cameras are good examples of this successful strategy. The right illumination is of paramount importance in light microscopy. TG provides fully system software-controlled solutions which are mostly maintenance free and have long lasting illuminantes. The correct filters which guarantee pixel shift-free and low channel crosstalk are equally important to make fluorescence scanning easily accessible for all users of a TG system.

TissueFAXS Scanning and Management software integrates all the hardware components into an easy to use workflow with full, walk-away automation capabilities.



TissueFAXS inverted Configuration

TissueFAXS iPlus LiveImaging

TMA Analysis with StrataQuest

Tissue microarrays (TMAs) have proven themselves as a standard molecular clinical diagnosis technique. TMAs are paraffin blocks in which up to 1000 individual tissue cores assembled in array fashion, allowing high-throughput histological analysis.

The TissueFAXS platform including the high-end image analysis solution StrataQuest provides specific workflow to scan and analyze the TMA samples containing multiple regions on one slide. TMA analysis in StrataQuest is available for images acquired in all imaging modalities (multispectral, fluorescence, brightfield).

In the first example, StrataQuest was utilized to analyze a TMA sample stained for hematoxylin (blue) and for E-cadherin present in the cell membranes of epithelial cells (brown). On the original image (a), E-cadherin is shown in brown and nuclei in blue. The aim was to detect the nuclei, the epithelial cells and the membranes of epithelial cells. The challenge with this analysis was that some cells do not contain nuclei.  

TMA Immune status Overview 15 name

Image (b) shows detected nuclei, covered by nuclei mask in light blue. Next only membranes were detected (c), which are shown in green; (d) shows the detection of the whole E-cadherin+ epithelial tissue, also marked in green.

 

Bild1 cut

In image (e) only cells with nuclei are identified, and the whole cell with the membrane is shown in blue, while the nuclei are covered by the light blue nuclei mask; (f) represents the nuclei in light blue and the total membranes in red.

 

Bild2 cut

Another example on TMA analysis is our IHC Immune Status in situ APP. Here the task was to develop a streamlined workflow for tissue classification (epithelium, stroma, lymphoid cluster).

Marker positive cells (for CD3+) were identified and spatial analysis was peformed, which would allow for the calculation of how many and which types of cells are located in defined distances from the object of interest (epithelium or lymphoid cluster). In the below example the App was applied to Tissue Microarrays (TMA) of an inflamed bladder.

 

TMA Immune status Overview 15 cut

One of the most notable features of StrataQuest is the machine learning-based tissue classification. To train the algorithm, the user marks regions assigned to different tissues and trains the classifier on the sample; later this workflow can be used on other samples. The following images represent the outcome of the classifier: (g) epithelium is detected in blue; (h) displays the lymphocyte cluster in light blue. These algorithms (or masks) can be combined to achieve a better visualization: (i) epithelium is displayed in blue, lymphocyte cluster in light blue and stroma in yellow.

 

TissueClassification cut

Beside the tissue classification itself, StrataQuest is capable of performing proximity analysis. It is possible to define specific proximity ranges; in the following case each range marked by different colors is 20µm. Nuclei detection was applied and CD3+ cells are shown in green, negative cells in yellow; (j) proximity ranges for the epithelium and (k) proximity ranges for the lymphocyte cluster.

 

TissueClassification2 cut

Apart from visual image analysis, StrataQuest outputs quantitative numbers. This way, it is possible to acquire and validate much more information. For example, StrataQuest can show how many cells are detected in each range: through the establishment of gates, 5.06% of CD3+ cells were found to be very close (in 20 µm range) to the epithelium.

 

scattergram Tumor Ranges edited cut

Further details about our various products and services can be found on our website. If you are interested in learning more about TissueGnostics solutions for TMA application or other tissue cytometry applications and our related services, please reach out to a member of the TissueGnostics team.

PROPERTIES

Design

Inverted microscope, modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Fluorescence and brightfield scanning

Compatible Slide Formats

Standard Slides, Over-sized Slides, Petri Dishes, Well-Plates

Slide Capacity

8

Objectives

Up to 6 Objectives (2.5-100x)

FL-Channels

5

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048px)
Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED
Solid-state Light Engine

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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