IHC 2 APP: Ki-67 nuclear staining analysis

Ki-67 staining and evaluation is an integral part of cancer diagnosis. Despite it being routinely used in pathology there still are issues to its correct use. Automated tissue analysis can provide a valuable supplement to visual evaluation. The IHC2 APP is among the StrataQuest easiest to use APPs and requires very little preparation time.

The first step of brightfield analysis is a color separation. Hematoxylin (blue) and Ki-67 (brown) stain are shown in separated grayscale images.

The nuclear segmentation algorithm is used on the nuclei, stained in hematoxylin and Ki-67. A virtual channel can be used for data from both stains.

Backward gating provides visual control of the correctness of the nuclear segmentation. This can be visualized both on the orginal and grayscale images.