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TissueFAXS Q & SL Q
CONFOCAL TISSUE CYTOMETERS
Full 1

TissueFAXS Q & SL Q Systems
Confocal Tissue Cytometer

TissueFAXS Q & SL Q Systems provide the benefits of both high-throughput of slides and laser-free fast confocal imaging. All TissueFAXS Fluo or PLUS system configurations, irrespective of whether they are upright or inverted, are also available as TissueFAXS Q configurations.

STissueFAXS Q systems are also available in scan only configurations.

 

TissueFAXS Q
KEY FEATURES

  • Whole slide confocal imaging
  • High-speed and high-resolution confocal imaging
  • LED-based technology
  • Multi-channel fluorescence & brightfield imaging
  • Inverted or upright
  • Upgrade SL Q: high-thoughput scanning - up to 120 slides 
  • Slide ID Scanner
  • Quantitative image analysis option
  • Default
  • Title
  • Date
  • Random

CONFIGURATION

TissueFAXS Q system comes in an upright, inverted, PLUS and slide loader (SL) configuration. The most advanced TissueFAXS Q system is the TissueFAXS SL Q+.

The TissueFAXS SL Q+ combines TG´s versatile Spinning Disc Confocal Kit with TissueFAXS SL autoloader capabilities and with TissueQuest/HistoQuest and/or StrataQuest image analysis software. 

TissueFAXS Q+ systems also provide all other scanning modes (i.e. brightfield and epifluorescence) of a non-confocal TissueFAXS system.

All epifluorescence TissueFAXS systems (upright and inverse) can be upgraded to TissueFAXS Q configuration at any time.

    TissueFAXS Q long

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

 IF_Tumor_Foci_Angio_overview-min.png

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

IF_Tumor_Foci_Angio_panel-min.png

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

IF_Tumor_Foci_Angio_scattergram.png

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

TF SLQ

   

TissueFAXS SL Q

The TissueFAXS SL Q is a highly flexible scanning platform, capable of scanning up to 120 slides at a time in brightfield, widefield fluorescence, and confocal fluorescence.

TissueFAXS Q delivers automated whole slide confocal imaging using a confocal spinning disk, paired with a high power LED light engine and the TissueFAXS automated scanning workflow. All TissueFAXS FL or PLUS systems, irrespective of whether they are upright or inverted, are also available in TissueFAXS Q configuration.

Upright configurations of the TissueFAXS Q can be upgraded with a 120 slide loader for high-throughput whole slide confocal imaging. Multiple types of projects - whether they are to be scanned in widefield fluorescence, or confocal fluorescence - can be easily color coded in the slide manager and scanned automatically with customizable scanning templates.

PROPERTIES

Design

Upright (Q+) or inverted (iQ+), Modular (Microscope, Cameras, Light-source, PC with 2 Monitors)

Microscopy mode

Confocal fluorescence, widefield fluorescence, (brightfield scanning - Q+ configuration)

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (standard configuration) or 120 (slide loader configuration)

Objectives

Up to 7 Objectives (2.5-100x)

FL-Channels

6

Camera

Monochrome: sCMOS camera (16-bit, 2048x2048, monochrome)
Color: CMOS camera (Q+ configuration)

Light Source

Solid-state light engine

Tissue Microarray (TMA)

YES

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - TissueQuest and HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: Integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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