TissueFAXS Q & SL Q Systems
Confocal Tissue Cytometer

TissueFAXS Q & SL Q Systems provide the benefits of both high-throughput of slides and laser-free fast confocal imaging. All TissueFAXS Fluo or PLUS system configurations, irrespective of whether they are upright or inverted, are also available as TissueFAXS Q configurations.

TissueFAXS Q systems are also available in scan only configurations.

CONFIGURATION

TissueFAXS Q system comes in an upright, inverted, PLUS and slide loader (SL) configuration. The most advanced TissueFAXS Q system is the TissueFAXS SL Q+.

The TissueFAXS SL Q+ combines TG´s versatile Spinning Disc Confocal Kit with TissueFAXS SL autoloader capabilities and with TissueQuest/HistoQuest and/or StrataQuest image analysis software.

TissueFAXS Q+ systems also provide all other scanning modes (i.e. brightfield and epifluorescence) of a non-confocal TissueFAXS system.

All epifluorescence TissueFAXS systems (upright and inverse) can be upgraded to TissueFAXS Q configuration at any time.

IF Dots App: Analysis of FISH

Fluorescence in situ hybridization (FISH) has been routinely used for decades in research and clinics to rapidly identify potential genetical changes within cells. This case study shows how TissueGnostics’ image analysis solution StrataQuest can be applied to evaluate FISH stainings. The most streamlined solution, the IF Dots App (runs within StrataQuest), can be applied to any analysis of dot-sized objects within the nuclei. Depending on the research needs, the following analysis can be combined with any other kind of analysis including AI-based tissue classification, phenotyping, spatial analysis, etc.

The aim of the following case study was to (i) identify individual nuclei and (ii) to assess how many FISH dots per individual nuclei are present.

The image below shows an overview of a triple staining: the nucleus stained by DAPI (blue), and two FISH markers, the housekeeping gene (green), and HER2/neu (red).

IF Dots original 95

The automated analysis starts with nuclei segmentation (a), where nuclei are identified by the intensity of the DAPI staining (nuclei are marked by the green outline). In the next step, the FISH dots are detected: in red the housekeeping gene in FITC channel (b) and in yellow for the HER2/neu in Acridine channel (c). Then these masks can be combined, as seen in (d).

IF Dots min

For further visualization of the analysis results, StrataQuest outputs the data in scattergrams. In the example below, the number of Acridine dots per nucleus (y-axis) is plotted against the FITC dots per nucleus (x-axis). The identified nuclei were gated for the condition “2 acridine dots per nuclei”, where the number of FITC dots can vary, and selected cells are shown in red contour via backward connection. Scattergrams showing the number of detected nuclei with each dot number combination are useful, especially with the Heat Map function enabled (bottom) which indicates those groups with a higher quantity of nuclei. To gain quantitative data for statistical analysis, the results can be immediately exported to Excel, CSV, and PDF formats.

IF Dots 2 min

FISH analysis is only one example of StrataQuest´s capabilities. If you are interested in how StrataQuest streamlined image analysis solution can enhance your research today, please reach out to a TissueGnostics team member for a demonstration.

Get to know more about FISH applications combined with TissueGnostics solutions in our blog posts:

TissueFAXS SL Q

The TissueFAXS SL Q is a highly flexible scanning platform, capable of scanning up to 120 slides at a time in brightfield, widefield fluorescence, and confocal fluorescence.

TissueFAXS Q delivers automated whole slide confocal imaging using a confocal spinning disk, paired with a high power LED light engine and the TissueFAXS automated scanning workflow. All TissueFAXS FL or PLUS systems, irrespective of whether they are upright or inverted, are also available in TissueFAXS Q configuration.

Upright configurations of the TissueFAXS Q can be upgraded with a 120 slide loader for high-throughput whole slide confocal imaging. Multiple types of projects - whether they are to be scanned in widefield fluorescence, or confocal fluorescence - can be easily color coded in the slide manager and scanned automatically with customizable scanning templates.