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TISSUE & CELL CULTURE CYTOMETERS
TissueFAXS i HISTO
Brightfield IMAGING
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TissueFAXS i Histo 
INVERTED BRIGHTFIELD SYSTEM

TissueFAXS i Histo is an inverted brightfield system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with HistoQuest or StrataQuest Histo image analysis software.

STissueFAXS i Histo is also available in a scan only configuration.

 

checkTissueFAXS i Histo can be upgraded to the TissueFAXS i PLUS configuration.

TissueFAXS i Histo
KEY FEATURES

  • Brightfield scanning
  • 8 slides scanning, petri dishes and well plates
  • Slide ID Scanner
  • Quantitative image analysis option
  • TMA & CISH scanning & analysis
  • Extended focus, stitching & illumination
  • Image and metadata storage, management and archiving
  • Default
  • Title
  • Date
  • Random

CONFIGURATION

TissueFAXS i Histo is TGs upright brightfield system. The system scans and analyses samples on slides, petri dishes and well plates in brightfield mode. The standard configuration comes equipped with an 8-slide stage. 

The objective turret allows for up to 7 objectives from all Zeiss objective classes with a M24 thread. TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. The TissueFAXS i Histo standard camera is a color 8 bit CMOS camera.

TissueFAXS Scanning and Management software integrates all the hardware components into an easy to use workflow with full, walk-away automation capabilities.

TissueFAXS i Histo diagonal

IHC 2 APP: Ki-67 Nuclear Staining Analysis

The nuclear protein Ki-67 is an established cell proliferation marker in research and clinics. Its staining and evaluation in tissue sections is an integral part of cancer diagnosis and prognosis.  Automated quantitative analysis of marker expression can provide a valuable supplement to visual evaluation. The IHC2 App is among the StrataQuest easiest-to-use analysis Apps and requires very little preparation time. This streamlined one-click analysis solution allows single cell detection, including the quantification of one additional marker within brightfield images.

The aim of the following case study was to assess (i) the total number of cells as well as (ii) the number and % of Ki-67 positive cells. (a) shows colon tissue stained for hematoxylin (blue) to detect the nuclei and Ki-67 (brown).

The first step of brightfield analysis is color separation. Using the color separation engine (b), the blue marker is automatically separated from the brown marker, as they are shown in grayscale images below.

The second step is the automatic identification of nuclei based on hematoxylin and Ki-67 staining. Since Ki-67 is located in the nuclei and is also covering the hematoxylin staining there, the nuclei can be detected from both markers. Analysis masks provide visual control of the correctness of the nuclear segmentation – here the nuclei are outlined in green (c).

After setting a cut-off on the intensity of the Ki-67 marker in the corresponding scattergram, Ki-67 positive cells are shown with a red outline (d).

IF Dots original 95

Backward connection allows to check cells of interest separately on the corresponding scattergram (e), for example, for checking a possible outlier. The scattergram on the example is plotted for mean Ki-67 intensity vs nuclei size. The results can be exported in common CSV or Excel formats.

 

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Nuclear segmentation, as well as marker detection, is only one of the possibilities of the streamlined analysis solution available for StrataQuest. Get inspired by other analysis solutions in our StrataQuest App Center. Our team would also be glad to develop a personalized solution for you.

PROPERTIES

Design

Inverted mircoscope, modular (microscope, camera, light-source, PC with 2 monitors)

Microscopy mode

Brightfield scanning

Compatible Slide Formats

Standard Slides, over-sized slides, petri dishes, well-plates

Slide Capacity

8

Objectives

Up to 6 Objectives (2.5-100x)

Camera

Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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