Full 1
TissueFAXS histo
UPRIGHT BRIGHTFIELD CYTOMETERS
Full 1

TissueFAXS Histo 
UPRIGHT BRIGHTFIELD SYSTEM

TissueFAXS Histo is an upright brightfield only system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays. The system is equipped with HistoQuest and/or StrataQuest Histo image analysis solftware.

STissueFAXS Histo is also available in a scan only configuration.

 

checkTissueFAXS Histo can be upgraded to the TissueFAXS PLUS configuration.

TissueFAXS Histo
KEY FEATURES

  • Brightfield scanning
  • Automated tissue detection
  • 8 slides scanning
  • Slide ID Scanner
  • Quantitative image analysis option
  • TMA & CISH scanning & analysis
  • Extended focus, stitching & illumination
  • Image and metadata storage, management and archiving
  • Default
  • Title
  • Date
  • Random

CONFIGURATION

TissueFAXS Histo is TG upright brightfield system. The system scans and analyses samples on slides and slide-based sample containers in brightfield mode. It comes equipped with an 8-slide stage. 

The objective turret allows for up to 7 objectives from all Zeiss objective classes with a M24 thread. TG continuously checks the market for cameras and other technical equipment to provide those solutions that deliver top performance and are cost-effective. Our current TissueFAXS Histo standard camera is a color 8 bit CMOS camera.

TissueFAXS Scanning and Management software integrates all the hardware components into an easy to use workflow with full, walk-away automation capabilities.

 TissueFAXS Histo Configuration

IF Dots App: Analysis of FISH

Fluorescence in situ hybridization (FISH) has been routinely used for decades in research and clinics to rapidly identify potential genetical changes within cells. This case study shows how TissueGnostics’ image analysis solution StrataQuest can be applied to evaluate FISH stainings.  The most streamlined solution, the IF Dots App (runs within StrataQuest), can be applied to any analysis of dot-sized objects within the nuclei. Depending on the research needs, the following analysis can be combined with any other kind of analysis including AI-based tissue classification, phenotyping, spatial analysis, etc.

The aim of the following case study was to (i) identify individual nuclei and (ii) to assess how many FISH dots per individual nuclei are present.

The image below shows an overview of a triple staining: the nucleus stained by DAPI (blue), and two FISH markers, the housekeeping gene (green), and HER2/neu (red).

IF Dots original 95

The automated analysis starts with nuclei segmentation (a), where nuclei are identified by the intensity of the DAPI staining (nuclei are marked by the green outline). In the next step, the FISH dots are detected: in red the housekeeping gene in FITC channel (b) and in yellow for the HER2/neu in Acridine channel (c). Then these masks can be combined, as seen in (d).

 

IF Dots min

For further visualization of the analysis results, StrataQuest outputs the data in scattergrams. In the example below, the number of Acridine dots per nucleus (y-axis) is plotted against the FITC dots per nucleus (x-axis). The identified nuclei were gated for the condition “2 acridine dots per nuclei”, where the number of FITC dots can vary, and selected cells are shown in red contour via backward connection. Scattergrams showing the number of detected nuclei with each dot number combination are useful, especially with the Heat Map function enabled (bottom) which indicates those groups with a higher quantity of nuclei. To gain quantitative data for statistical analysis, the results can be immediately exported to Excel, CSV, and PDF formats.

 

IF Dots 2 min

FISH analysis is only one example of StrataQuest´s capabilities. If you are interested in how StrataQuest streamlined image analysis solution can enhance your research today, please reach out to a TissueGnostics team member for a demonstration.

Get to know more about FISH applications combined with TissueGnostics solutions in our blog posts:

PROPERTIES

Design

Upright microscope, modular (microscope, camera, light-source, PC with 2 monitors)

Microscopy mode

Brightfield scanning

Compatible Slide Formats

All standard and over-sized slides

Slide Capacity

8 (upgrade option to TissueFAXS Histo SL for automated scanning of 120 slides)

Objectives

Up to 7 Objectives (2.5-100x)

Camera

Color: CMOS camera (8-bit, 2048x2048px)

Light Source

VIS-LED

Tissue Microarray (TMA)

Yes

Image Analysis Software

contextual image analysis - StrataQuest, StrataQuest Apps
single cell analysis - HistoQuest

Supported Image Formats

TissueFAXS, OME-TIFF, TIFF, JPEG, BMP, PNG

Viewer

TissueFAXS Viewer: integrated, stand-alone freeware

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies).
You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.