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StrataFAXS II
COMPACT BRIGHTFIELD CYTOMETER
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StrataFAXS II 
Streamlined Tissue Cytometry

StrataFAXS II is an upright brightfield slide scanner for acquisition and analysis of HE/IHC processed tissue sections, smears and Tissue Microarrays (TMA). StrataFAXS II with its innovative workflow allows you to directly work on your samples via real-time scanning and live stitching. The system is equipped with HistoQuest single cell analysis software and/or StrataQuest contextual image cytometry software which includes a machine learning module for automated classification of histological entities (e.g. tumor foci, epidermis, glands, glomeruli) and a deep neural network for precise nuclear segmentation even at high cellular densities and low expression levels.

StrataFAXS II
KEY FEATURES

  • Instantly navigate across slides with real-time scanning
  • Live stitching
  • Brightfield mode
  • 2 slide scanning
  • Live view and annotate samples
  • Quantitative image analysis solutions (StrataQuest, HistoQuest)
  • Easy-to-use design and easy-to-clean horizontal stage

StrataFAXS II - Whole slide imaging 

 StrataFAXS II MouseEmbryo1

 

StrataFAXS II MouseEmbryo2

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

 IF_Tumor_Foci_Angio_overview-min.png

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

IF_Tumor_Foci_Angio_panel-min.png

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

IF_Tumor_Foci_Angio_scattergram.png

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

StrataFAXS II - Analysis

Use TissueGnostics' analysis soltuion StrataQuest with the integrated machine learning-based Classifier to automatically segment the tissue section into different tissue entities. 

StrataFAXS II Ratjoint Classifier Part1 1000StrataFAXS II Ratjoint Classifier Part2 1000  

PROPERTIES

Special features

three operating modes: Instant Scan, Live, and Slide Scan

Design

Compact scanner, integrated brightfield camera and light-source, PC with 2 Monitors

Scanner stand

Upright

Scanning mode

Brightfield scanning

Compatible slide formats

Standard and double-sized slides

Slide capacity

2 (1 double-sized)

Supported objectives

20x, 40x, 60x air

Light source

White light LED

Image Analysis Software

StrataQuest (contextual tissue cytometry), StrataQuest brightfield APPsHistoQuest (singel-cell analysis)

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

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