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IHC Meta.Cells APP: Stroma and tumor detection on morphological stains

One of the main strengths of StrataQuest is the capability to segment morphological structures based on a morphological stain alone (i.e. without a specific staining of the structure to be detected).

In this case, the task was made more difficult due to many very thin areas of stroma. The tumor sample was stained immunohistochemically for CD8+ T-cells (brown) and with a hematoxylin counterstain (blue). The counterstain was separation of tumor and stroma. It was shown that 17.3% of the sample are stroma (orange outline). 22% of CD8+ T-cells are located in stroma areas (outlined in green). Outlined in red are CD8+ cells located in the tumor area.

stromaresultfull

 

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