EVENTS

 

MAY
2023

The Korean Society of Pathologists Spring Conference, DAEGU (SK)

First International Societies For Investigative Dermatology Meeting, TOKYO (JP)

CYTO 2023, MONTREAL (CA)

China Life Science Conference, GUANGZHOU (CN)

JUNE
2023

Annual Meeting of the Brazilian Society of Biochemistry and Molecular Biology, Águas de Lindóia (BR)

Redox Biology in Translation, VIENNA (AU)

The 49nd Meeting Korean Cancer Association, SEOUL (SK)

EMBO Workshop: Imaging the Immune system, LUGANO (CH)

JULY
2023

Microscience Microscopy Congress (MMC), MANCHESTER (UK)

Analytica China, SHANGHAI (CN)

SEPTEMBER
2023

The Korean Society for Brain and Neural Sciences (KSBNS), BUSAN (SK)

Asian Federation of Laboratory Animal Science Associations Congress (AFLAS), JEJU (SK)

Korean Association Of Immunologists International Conference, SEOUL (SK)

Digital Pathology Seoul, SEOUL (SK)

OCTOBER
2023

The Korean Society of Pathologists 2023 Fall Conference, SEOUL (SK)

NOVEMBER
2023

The Korean Society for Molecular and Cellular Biology (KSMCB), SEOUL (SK)

Spatial Biology Singapore, SINGAPORE

Korean Society for Extracellular Vesicles (KSEV), JEJU (SK)

22nd International Conference on Bioinformatics (InCoB 2023), BRISBANE (AUT)

Adipocyte App: Measurement of Cellular Size of Adipocytes

The StrataQuest-based Adipocyte App was applied to this sample with the aim to automatically detect (i) the number, (ii) the size, and (iii) the diameter of adipocytes within the given fat tissue. The challenge of analyzing the adipocytes is that the nuclei cannot be detected properly since they are either usually washed away during the H&E staining process, or are not visible. Therefore, the App detects the adipocytes using the membranes of the cells.

(a) Shows fat tissue stained with hematoxylin & eosin (H&E). The App first detects the membranes of the adipocytes and minor membrane tears are automatically closed (b).  To detect the individual cells without nuclei, the App uses the detected membranes as a starting point and defines the cell by its distance from the membrane (c). Here the final result of cell detection is shown (d), cells are labeled in different colors for visualization purposes.

The App automatically eliminates adipocytes on the borders from the analysis which may influence the proper outcome of the analysis.

 IF_Tumor_Foci_Angio_overview-min.png

The results of the analysis are visualized in histograms and scattergrams. As shown in the scattergram below, the cells can be grouped according to their diameter (e) or (f) size.

IF_Tumor_Foci_Angio_panel-min.png

By selecting a gate of interest within the scattergram and using the backward connection feature, the cells from the selected gate are viewed directly in the sample in their native tissue environment (g). In this case, cells with diameters of 120µm to 129µm are shown in red.

IF_Tumor_Foci_Angio_scattergram.png

The gained measurements can be exported from StrataQuest in CSV and /or Excel format.

StrataQuest Apps offer a streamlined and easy workflow for analyzing your samples. Look through our StrataQuest Apps and get inspired by the variety of biomedical image analysis applications. If you cannot find the perfect solution for your research question, please contact us. TissueGnostics offers the development of customized Apps for your specific analysis needs.

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
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