Analysis of cyclic stain and bleach samples

Analysis of cyclic stain and bleach samples

Multi-epitope ligand cartography (MELC) is a technology using samples subjected to cycles of fluorescent staining, imaging and photobleaching. Each cycle can use an antibody for a different protein. The result is a set of images of the distributions of many proteins for the same samples.

Tumor areas are detected using anti-cytokeratin+ cells and their density. Blood vessels are detected using anti-CD31 + structures. The distance of tumor cells from blood vessels within the tumor areas can then be measured.

A specialized APP was established to analyze a MELC sample of skin from a melanoma patient. The aim was to quantify the expression of all 90 proteins in close proximity to Melan-A positive areas. Some of the resulting images are shown below.

StrataQuest is ideal to fully address the immense potential of such samples with automated context analysis.


The possibilities of the combination of cyclic stain-bleach methods and StrataQuest context-based analysis are almost endless.

Which protein increases in its expression density and which one decreases in relation to its distance to:

Normal melanocytes, Melanoma cells, Blood vessels, Inflammation sites, Sweat glands, Hair follicles, Basal membrane, Langerhans cells, Intraepithelial T-cells, Regulatory T-cells? 


Question head 70x70Want to know more about StrataQuest capabilities?

Please just let us know.


GDPR Icon2 attention 

By contacting TissueGostics for information via the mail link above please note that you consent to us processing any personal data submitted in your information request.

Pursuant to the requirements of the General Data Pretection Regulation (EU) we will process your data as long as needed to provide you with the requested information, after which your data will be restricted, .i.e. locked and not processed for other purposes.

Your consent to us processing your data can be revoked at any time - please inform us about this via e-mail to

For further information see our Privacy and Data Protection Policy

zh cn
Cookies make it easier for us to provide you with our services. With the usage of our services you permit us to use cookies.
More information Ok