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About us
Tissuegnostics
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CREATING THE RIGHT SPIRIT

TissueGnostics is an innovative enterprise on the cutting edge of science and technology our activities are guided by economic principles. We provide our customers with a new generation of imaging and analysis tools for basic and clinical research.

We offer objectivity, quality and innovation and continuously demand it from ourselves. Through const professional development, We maintain top-level scientific standards and competence by constantly reviewing and improving our processes, both scientific and business.

Hallmarks of our work include creativity, commitment and mutual understanding at all levels of operation. We make sure that our staff members enjoy a pleasant working environment that brings out their best performance.

FOCUSING ON PEOPLE

People are the focus of our work, whether they are patients, researchers, customers or staff members. We redefine ourselves constantly by building relationships between people and finding for the requirements of people.

EXCHANGING THOUGHTS

Everyday communication is about people exchanging ideas. We promote sincere communication in an atmosphere of trust and mutual respect to further creativity, satisfaction and social competence.

We are closely cooperating with clinicians and research facilities to ensure that our development work will always be customer-oriented. Custom requests and needs expressed by individuals are being incorporated to gear our products for a broad spectrum of challenges.

JOINING FORCES

We are making every effort to promote a sense of community enabling us to act as a team.

Our daily interactions are guided by mutual respect and pervaded by a peer-to-peer spirit. We strongly believe that social competence should not be a hollow phrase. Therefore we practice what we preach.

Our efforts are supported by solid training, continuous professional development, setting targets, and being responsible individuals. We cannot accept poor working conditions.

TMA Analysis with StrataQuest

Tissue microarrays (TMAs) have proven themselves as a standard molecular clinical diagnosis technique. TMAs are paraffin blocks in which up to 1000 individual tissue cores assembled in array fashion, allowing high-throughput histological analysis.

The TissueFAXS platform including the high-end image analysis solution StrataQuest provides specific workflow to scan and analyze the TMA samples containing multiple regions on one slide. TMA analysis in StrataQuest is available for images acquired in all imaging modalities (multispectral, fluorescence, brightfield).

In the first example, StrataQuest was utilized to analyze a TMA sample stained for hematoxylin (blue) and for E-cadherin present in the cell membranes of epithelial cells (brown). On the original image (a), E-cadherin is shown in brown and nuclei in blue. The aim was to detect the nuclei, the epithelial cells and the membranes of epithelial cells. The challenge with this analysis was that some cells do not contain nuclei.  

TMA Immune status Overview 15 name

Image (b) shows detected nuclei, covered by nuclei mask in light blue. Next only membranes were detected (c), which are shown in green; (d) shows the detection of the whole E-cadherin+ epithelial tissue, also marked in green.

 

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In image (e) only cells with nuclei are identified, and the whole cell with the membrane is shown in blue, while the nuclei are covered by the light blue nuclei mask; (f) represents the nuclei in light blue and the total membranes in red.

 

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Another example on TMA analysis is our IHC Immune Status in situ APP. Here the task was to develop a streamlined workflow for tissue classification (epithelium, stroma, lymphoid cluster).

Marker positive cells (for CD3+) were identified and spatial analysis was peformed, which would allow for the calculation of how many and which types of cells are located in defined distances from the object of interest (epithelium or lymphoid cluster). In the below example the App was applied to Tissue Microarrays (TMA) of an inflamed bladder.

 

TMA Immune status Overview 15 cut

One of the most notable features of StrataQuest is the machine learning-based tissue classification. To train the algorithm, the user marks regions assigned to different tissues and trains the classifier on the sample; later this workflow can be used on other samples. The following images represent the outcome of the classifier: (g) epithelium is detected in blue; (h) displays the lymphocyte cluster in light blue. These algorithms (or masks) can be combined to achieve a better visualization: (i) epithelium is displayed in blue, lymphocyte cluster in light blue and stroma in yellow.

 

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Beside the tissue classification itself, StrataQuest is capable of performing proximity analysis. It is possible to define specific proximity ranges; in the following case each range marked by different colors is 20µm. Nuclei detection was applied and CD3+ cells are shown in green, negative cells in yellow; (j) proximity ranges for the epithelium and (k) proximity ranges for the lymphocyte cluster.

 

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Apart from visual image analysis, StrataQuest outputs quantitative numbers. This way, it is possible to acquire and validate much more information. For example, StrataQuest can show how many cells are detected in each range: through the establishment of gates, 5.06% of CD3+ cells were found to be very close (in 20 µm range) to the epithelium.

 

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Further details about our various products and services can be found on our website. If you are interested in learning more about TissueGnostics solutions for TMA application or other tissue cytometry applications and our related services, please reach out to a member of the TissueGnostics team.

HISTORY

TissueGnostics was established in 2003 in Austria after nearly a decade of basic research. The goal was to supply scientists with qualitative and observer independent results for cell and tissue analysis in tissue context and this in automated format and significantly less time. Katja Österreicher, Georg Steiner and Dr. Rupert Ecker, the three co-founders of the company, with the aid of a dozen of development partners (Medical University Vienna, Memorial Sloan-Kettering Cancer Center New York, Ludwig Boltzmann Institute for Cancer Research, Vienna; Fred Hutchinson Cancer Center, Seattle; Brighamand Women`s Hospital, Boston; etc.) succeeded in reaching this target by 2005.

The product launch of TissueFAXS 1.0 was in 2006 and provided tissue imaging and automated identification and functional characterization of single cells in tissue sections. In contrast to morphometry, which provides values referring to the metric dimensions of cells, the term “tissue cytometry” refers to quantification of molecular parameters. Though methodically different, tissue cytometry exhibits a functional similarity to flow cytometry. While flow cytometer is restricted to cells in suspension (e.g. blood) and cannot be applied to solid tissue, tissue cytometry refers to the cytometric analysis (as opposed to morphometric analysis) of histological sections. TissueGnostics has been the first manufacturer of tissue cytometers offering a flow cytometry-like workflow (but applied to tissue sections).

Today in 2022 TissueGnostics is a successfull company with subsidiaries in the EU, the USA and China and with costumers on all continents.
 

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
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