• 1

WELCOME

TissueGnostics provides fully integrated cutting-edge tissue cytometers for (i) whole-slide imaging (brightfield, fluorescence, confocal, multispectral) and (ii) high-end analysis of tissue sections, cultured cells, TMAs, smears etc. Explore TGs solutions for spatial phenotyping, single cell analysis, molecular single cell profiling, machine-learning based tissue classification and many more.
 

IHC 2 APP: Ki-67 Nuclear Staining Analysis

The nuclear protein Ki-67 is an established cell proliferation marker in research and clinics. Its staining and evaluation in tissue sections is an integral part of cancer diagnosis and prognosis.  Automated quantitative analysis of marker expression can provide a valuable supplement to visual evaluation. The IHC2 App is among the StrataQuest easiest-to-use analysis Apps and requires very little preparation time. This streamlined one-click analysis solution allows single cell detection, including the quantification of one additional marker within brightfield images.

The aim of the following case study was to assess (i) the total number of cells as well as (ii) the number and % of Ki-67 positive cells. (a) shows colon tissue stained for hematoxylin (blue) to detect the nuclei and Ki-67 (brown).

The first step of brightfield analysis is color separation. Using the color separation engine (b), the blue marker is automatically separated from the brown marker, as they are shown in grayscale images below.

The second step is the automatic identification of nuclei based on hematoxylin and Ki-67 staining. Since Ki-67 is located in the nuclei and is also covering the hematoxylin staining there, the nuclei can be detected from both markers. Analysis masks provide visual control of the correctness of the nuclear segmentation – here the nuclei are outlined in green (c).

After setting a cut-off on the intensity of the Ki-67 marker in the corresponding scattergram, Ki-67 positive cells are shown with a red outline (d).

IF Dots original 95

Backward connection allows to check cells of interest separately on the corresponding scattergram (e), for example, for checking a possible outlier. The scattergram on the example is plotted for mean Ki-67 intensity vs nuclei size. The results can be exported in common CSV or Excel formats.

 

IF Dots min

Nuclear segmentation, as well as marker detection, is only one of the possibilities of the streamlined analysis solution available for StrataQuest. Get inspired by other analysis solutions in our StrataQuest App Center. Our team would also be glad to develop a personalized solution for you.

 
  • TG AUSTRIA
    Global Headquarter

    Taborstrasse 10/2/8
    A-1020 Vienna
    AUSTRIA, EU

    Tel.: +43/1/216 11 90

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    TG ROMANIA

    Str. Sf. Andrei, nr.15A
    700028 Iasi
    ROMANIA, EU

    Tel.: +40/332/40 58 66

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Read More
  • TG USA 

    12522 Moorpark Street
    Suite #106
    Los Angeles, CA 91604
    USA

    Tel.: +1/818/856/8056

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    TG USA East

    ScientiaLux d.b.a.
    4 Farnum Terrace
    Worcester, MA 01602
    USA

    Tel.: +1/508/471/7732

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Read More
  • TG ASIA PACIFIC

    China Division
    Room 506,
    No.6 Auto Museum East Rd,
    Fengtai District Beijing
    CHINA

    Tel.: +86/400/898 1980

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    Western Pacific Division
    Taipei

    Tel.: +886/928/899 397

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    Australia Division
    Brisbane, Queensland

    Tel.: +61/416 037 618

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Read More
  • TG AFRICA

    Measuring Instruments Technology (MIT)
    CSIR Campus, Building 33
    Meiring Naude Rd
    Brummeria, Pretoria 0181
    SOUTH AFRICA

    Tel.: +27 12 349 5191
    Cell: +27 82 784 2011

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Read More
  • 1

Contact

TissueGnostics GmbH
Taborstraße 10/2/8
1020 Vienna, Austria
+43 1 216 11 90
This email address is being protected from spambots. You need JavaScript enabled to view it.

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies).
You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.