IHC Immune Status in situ APP: Tumor immunology

IHC Immune Status in situ APP: Tumor immunology

The IHC Immune Status in situ APP is ideally suited for immunological investigations due to its capability to measure the distance of single cells from tissue metastructures.

In this example this is applied to bladder cancer tumor sections on Tissue Microarrays (move mouse over the images).

In this TMA core DAB stained CD3 positive lymphocytes are evaluted in the context of their distance from automatically detected tumor regions as well as from large and small lymphocyte clusters.

TMA core with tumor detection 
and tumor proximity ranges                          

lymphocyte applymphocyte app

TMA core with lymphocyte cluster detection 
and lymphocyte cluster proximity ranges                         

lymphocyte applymphocyte app

Lymphocytes (CD3+, purple contour) within tumor proximity
                                                                                                                          

lymphocyte applymphocyte app

Lymphocytes (CD3+, purple contour) within
lymphocyte cluster proximity                                    

lymphocyte applymphocyte app

   

 Sample courtesy of Dr. Kathleen Williamson, Centre for Cancer Research and Cell Biology, Queen’s University, Belfast

 

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