IHC Membrane APP: HER2/neu in Breast Cancer

IHC Membrane APP: HER2/neu in Breast Cancer

The aim of the project was to quantify the distribution of anti-CD3 positive cells in and around the epithelial areas of prostate biopsies from a clinical study.

The biopsies were provided by the Medical University of Vienna.

The main diagnostic requirements are to measure the intensity of the HER2/neu membrane staining and the completeness of the closure of the respective membrane rings. The IHC Membrane APP outputs this as the "angle of staining" parameter in degrees, as shown in the following scttergram (high angles of staining in the blue gate).

Backward Gating for all cells showing

The following images show some results of steps in the automated analysis process.

Result of automated color separation 
for HER2/neu staining

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HER2/neu membrane detection algorithm results,

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Automatic nuclear segmentation algorithm results

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Tumor nuclei selected (red contour vs. green) in  
strong HER2/neu areas

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Low angle of HER2/neu staining, low intensity

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High angle of HER2/neu staining, high intensity

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