IF Immune Status in situ APP: Distance of tumor cells from blood vessels

IF Immune Status in situ APP: Distance of tumor cells from blood vessels

The following example was analysed using a combination of the IF Immune Status in siu APP and the FL version of the Angio APP. It was done for the Johns Hopkins Hospital in New York.

The aim was to evaluate the distance of tumor cells from blood vessels within the tumor cell clusters.

Tumor areas are detected using anti-cytokeratin+ cells and their density. Blood vessels are detected using anti-CD31 + structures. The distance of tumor cells from blood vessels within the tumor areas can then be measured.

Some basic steps of the analysis are shown below.

brain tumor digital slaide

Brain tumor whole digital slide


Enlarged

NucSec

TumRegs
 Detail view  Nuclear segmentation  Tumor areas segmentation
 TumReg BV  AllSegs  DistTrans
 Tumor areas & blood vessels, cleaned  All detected objects   BV Distance Transformation masks
 DistRanges  0 50  50 100
 Dist. ranges, 0-50, 50-100, 100-150 μm  Nuclei within 0-50μm range  Nuclei within 50-100μm range

 

 Final result: Number of tumor cells within each distance range of a blood vessel: 0-50 μm: 6.988/52%; 50-100 μm: 3.483/26%; 100-150 μm: 1.845/14%

 

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